MUC5AC 抗体
Quick Overview for MUC5AC 抗体 (ABIN6937959)
抗原
See all MUC5AC 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the N-terminal cysteine rich part of the peptide core of MUC5AC, which is heavily glycosylated. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. MAb 2-11M1 reacts with the protein backbone exclusively, it only reacts with fully deglycosylated MUC5AC. Therefore, the material under test should also be fully deglycosylated. This can be achieved with standard periodate oxidation method. The success of the deglycosylation can be checked with routine PAS (Periodic Acid Shiff) staining. After deglycosylation, the preparation should no longer be stainable with PAS reagent. Only then 2-11M1 will react should MUC5AC be present. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
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亚型
- IgG1 kappa
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anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 5528-5627) antibody
MUC5AC 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 8H8G6 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) antibodyMUC5AC 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 28-327) antibodyMUC5AC 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 6C9D11 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) antibodyMUC5AC 适用: 人, 小鼠, 大鼠, 猴, Cat, 兔, 小鸡, Pig, Hedgehog IHC, StM 宿主: 小鼠 Monoclonal 45M1 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 5528-5627) antibodyMUC5AC 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 7E9D8 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 601-700) antibodyMUC5AC 适用: 人, 小鼠, 大鼠 ELISA, ICC, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 430-549) antibodyMUC5AC 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 351-450) antibodyMUC5AC 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H7 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) antibodyMUC5AC 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-109M unconjugated Recombinant Antibody
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (C-Term) antibodyMUC5AC 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA) Optimal dilution for a specific application should be determined.
Positive_Control: Human colon or stomach (IHC).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))
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别名
- MUC5AC
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背景
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Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM),MUC5AC (Mucin 5AC / Gastric Mucin)
Cellular localisation: Cytoplasmic -
分子量
- >1,000kDa
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基因ID
- 4586, 534332
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UniProt
- P98088
抗原
-
