MUC5AC 抗体
Quick Overview for MUC5AC 抗体 (ABIN6937958)
抗原
See all MUC5AC 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This MAb recognizes the peptide core of gastric mucin M1 (1,000 kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
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无交叉反应
- 牛
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交叉反应 (详细)
- Human. Monkey. Rabbit. Cat. Mouse. Rat. Pig. Hedgehog. Chicken. Does not react with Cow.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
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亚型
- IgG1 kappa
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Human colon or stomach (IHC).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))
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别名
- MUC5AC
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背景
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Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM),MUC5AC (Mucin 5AC / Gastric Mucin)
Cellular localisation: Cytoplasmic -
分子量
- >1,000kDa
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基因ID
- 4586, 534332
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UniProt
- P98088
抗原
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