Recombinant Cytokeratin 18 抗体
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北京 101111
Quick Overview for Recombinant Cytokeratin 18 抗体 (ABIN6937689)
抗原
See all Cytokeratin 18 (KRT18) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Recombinant Cytokeratin 18 (KRT18) Antibody
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特异性
- This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
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无交叉反应
- 小鸡, 非洲爪蟾
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交叉反应 （详细）
- Human. Mouse. Rat. Hamster. Ferret. Cow. Pig. Cat. Dog. Sheep. Goat. Horse. Marmoset monkey. Does not react with Chicken or Xenopus.
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纯化方法
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant human full-length KRT18 protein
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亚型
- IgG, kappa
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anti-Keratin 18 (KRT18) (AA 53-150) antibody
KRT18 适用: 人, 小鼠, 大鼠, Pig WB, FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) antibodyKRT18 适用: 哺乳动物 WB, ELISA, IHC (p), ICC, IP, ICFC 宿主: 小鼠 Monoclonal C-04 unconjugated
anti-Keratin 18 (KRT18) antibodyKRT18 适用: 人 WB, ELISA, IHC (p), ICC, IP, ICFC 宿主: 小鼠 Monoclonal DC-10 unconjugated
anti-Keratin 18 (KRT18) (AA 1-423) antibodyKRT18 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 238-396) antibodyKRT18 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 238-396) antibodyKRT18 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal E4 unconjugated
anti-Keratin 18 (KRT18) (AA 72-380) antibodyKRT18 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 1-430) antibodyKRT18 适用: 人 WB, IF, ELISA, IHC (p), IP 宿主: 小鼠 Monoclonal 2F8 unconjugated
anti-Keratin 18 (KRT18) (AA 102-396) antibodyKRT18 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 69-372) antibodyKRT18 适用: 人, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5E8 unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells),Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HCT116, MCF-7, HeLa cells, Breast Cancer.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
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别名
- KRT18
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背景
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Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18
Breast cancer., HCT116, HeLa cells, MCF-7, Human Intestine, Human Liver, Human Lung, A431
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分子量
- 45kDa
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基因ID
- 3875, 406013
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UniProt
- P05783
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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