KRT 抗体
Quick Overview for KRT 抗体 (ABIN6937637)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 55 kDa (CK7), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 46 kDa (CK17), 45 kDa (CK18) and 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad-spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.
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交叉反应 （详细）
- Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Cat and Chicken.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Human epidermal keratins
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亚型
- IgG1 kappa
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anti-Keratin 126 Pseudogene (KRT126P) antibody
KRT126P 适用: 人, 犬, Cat FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal PCK-3150 unconjugated
anti-Keratin 126 Pseudogene (KRT126P) antibodyKRT126P 适用: 人, 小鼠, 大鼠, 犬, 小鸡, Cow, 猴, 兔 FACS, IHC, StM 宿主: 小鼠 Monoclonal Cocktail PAN-CK unconjugated
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应用备注
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Known_Application: Flow Cytometry (0.5-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-2 μg/mL for 2 hours at RT),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, MCF-7, Skin, Adeno- or Squamous carcinomas
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))
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别名
- KRT
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背景
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K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39,Cytokeratin, pan (Epithelial Marker)
Cellular localisation: Cytoplasmic -
分子量
- 40-67kDa (Multiple)
抗原
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