Recombinant KRT 抗体
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北京 101111
Quick Overview for Recombinant KRT 抗体 (ABIN7127255)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Recombinant Cytokeratin, pan (Epithelial Marker) Antibody
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特异性
- Anti-cytokeratin clone rPCK/6750 (CK rPCK/6750) demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, rPCK/6750 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, rPCK/6750 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.
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交叉反应 （详细）
- Human, Dog and Cat.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Crude cytokeratin extract prepared from RT-4 and MCF-7 cells.
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亚型
- IgG1, kappa
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anti-Keratin 126 Pseudogene (KRT126P) antibody
KRT126P 适用: 人, Cat, 犬 IHC, FACS, IF 宿主: 小鼠 Monoclonal PCK-3150 unconjugated
anti-Keratin 126 Pseudogene (KRT126P) antibodyKRT126P 适用: 人, 小鼠, 大鼠, Cat, 犬, 小鸡, Cow, 猴, 兔 WB, IHC, FACS, IF 宿主: 小鼠 Monoclonal Cocktail PAN-CK unconjugated
anti-Keratin 126 Pseudogene (KRT126P) (acLys194) antibodyKRT126P 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Keratin 126 Pseudogene (KRT126P) antibodyKRT126P 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Skin, adeno- or squamous carcinomas (IHC).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))
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别名
- KRT
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背景
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K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39
adeno- or squamous carcinomas (IHC)., Skin, A431.
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分子量
- 40-67kDa ()
抗原
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