KRT 抗体
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北京 101111
Quick Overview for KRT 抗体 (ABIN6941353)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Cytokeratin, pan (Epithelial Marker) Antibody
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特异性
- Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 55 kDa (CK7), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 46 kDa (CK17), 45 kDa (CK18) and 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad-spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Human epidermal keratins
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亚型
- IgG, kappa
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anti-Keratin 126 Pseudogene (KRT126P) antibody
KRT126P 适用: 人, Cat, 犬 IHC, FACS, IF 宿主: 小鼠 Monoclonal PCK-3150 unconjugated
anti-Keratin 126 Pseudogene (KRT126P) (acLys194) antibodyKRT126P 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Keratin 126 Pseudogene (KRT126P) antibodyKRT126P 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Positive Control: HeLa, MCF-7, Skin, Adeno- or Squamous carcinomas.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))
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别名
- KRT
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背景
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K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39
Adeno- or Squamous carcinomas, HeLa, MCF-7, Skin
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分子量
- 40-67kDa ()
抗原
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