CD40 Ligand 抗体 (AA 108-261)
Quick Overview for CD40 Ligand 抗体 (AA 108-261) (ABIN6936712)
抗原
See all CD40 Ligand (CD40LG) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 108-261
-
特异性
- CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells.
-
交叉反应 （详细）
- Human.
-
纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
-
免疫原
- Recombinant fragment of human CD40LG protein (around aa 108-261) (exact sequence is proprietary)
-
亚型
- IgG1 kappa
-
-
anti-CD40 Ligand (CD40LG) (AA 1-261) antibody
CD40LG 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD40 Ligand (CD40LG) (AA 108-261) antibodyCD40LG 适用: 人 FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal CD40LG-2763 unconjugated
anti-CD40 Ligand (CD40LG) (AA 108-261) antibodyCD40LG 适用: 人 FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal CD40LG-2761 unconjugated
anti-CD40 Ligand (CD40LG) antibody (FITC)CD40LG 适用: 小鼠 FACS 宿主: 仓鼠 Monoclonal MR-1 FITC
anti-CD40 Ligand (CD40LG) (AA 79-129) antibodyCD40LG 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD40 Ligand (CD40LG) (AA 33-62) antibodyCD40LG 适用: 人 WB, FACS, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-CD40 Ligand (CD40LG) (AA 111-261) antibodyCD40LG 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD40 Ligand (CD40LG) antibody (PE)CD40LG 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 24-31 PE
anti-CD40 Ligand (CD40LG) (AA 47-261) antibodyCD40LG 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 8H10F5 unconjugated
anti-CD40 Ligand (CD40LG) (AA 47-261) antibodyCD40LG 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 5A3A9 unconjugated
-
-
应用备注
-
Known_Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat cells. Human tonsil, spleen or thymus tissue (IHC).
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
-
储存液
- Azide free
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
-
有效期
- 24 months
-
-
- CD40 Ligand (CD40LG)
-
别名
- CD40LG
-
背景
-
CD154, gp39, hCD40L, HIGM1, IGM, IMD3, T B cell activating molecule, T BAM, T-cell antigen Gp39, TNF-related activation protein,TrAP, Tumor necrosis factor ligand superfamily member 5 (TNFSF5),CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes)
Cellular localisation: Cell surface. Secreted. -
分子量
- 36kDa
-
基因ID
- 959, 592244
-
UniProt
- P29965
-
途径
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
-
