CD3 epsilon 抗体 (Cytoplasmic Domain)
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- 抗原 See all CD3 epsilon (CD3E) 抗体
- CD3 epsilon (CD3E)
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抗原表位
- AA 156-168, Cytoplasmic Domain
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD3 epsilon antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
- 交叉反应 （详细）
- Human, Rat, Guinea pig, Rabbit, Pig, Horse, Cow and Chicken. Others not known.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3- chain
- 克隆位点
- PC3-188A
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CD3E Primary Antibody
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anti-CD3 epsilon (CD3E) (AA 23-207) antibody
CD3E 适用: 人, 小鼠, 大鼠 WB, IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 156-168), (Cytoplasmic Domain) antibodyCD3E 适用: 人, Pig, 大鼠, 兔, Cow, 马, 豚鼠, 小鸡 FACS, WB, IF 宿主: 小鼠 Monoclonal PC3-188A unconjugated
anti-CD3 epsilon (CD3E) (AA 23-119) antibodyCD3E 适用: 人 FACS, WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal C3e-1931 unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E 适用: 人, 小鼠, 大鼠 FACS, IF, Func 宿主: 小鼠 Monoclonal RIV9 unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 25-207) antibodyCD3E 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 28-185) antibodyCD3E 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 23-207) antibodyCD3E 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Known_Application: Flow Cytometry (0.5-1 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Western Blotting (1-2 μg/mL for 60 minutes at RT),Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat cells. Tonsil and lymph node
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- CD3 epsilon (CD3E)
- 别名
- CD3E (CD3E 产品)
- 背景
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CD 3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex,CD3e (T-Cell Marker)
Cellular localisation: Cell Surface and Cytoplasmic - 分子量
- 20kDa
- 基因ID
- 916, 3003
- UniProt
- P07766
- 途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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