CD3 epsilon 抗体 (Cytoplasmic Domain)
Quick Overview for CD3 epsilon 抗体 (Cytoplasmic Domain) (ABIN6936685)
抗原
See all CD3 epsilon (CD3E) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 156-168, Cytoplasmic Domain
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特异性
- Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
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交叉反应 （详细）
- Human, Rat, Guinea pig, Rabbit, Pig, Horse, Cow and Chicken. Others not known.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3- chain
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亚型
- IgG1 kappa
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anti-CD3 epsilon (CD3E) (AA 23-207) antibody
CD3E 适用: 人, 小鼠, 大鼠, 小鸡 WB, IHC, IF, IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E 适用: 人, 小鼠, 大鼠 FACS, IF, Func 宿主: 小鼠 Monoclonal RIV9 unconjugated
anti-CD3 epsilon (CD3E) (AA 23-119) antibodyCD3E 适用: 人 FACS, WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal C3e-1931 unconjugated
anti-CD3 epsilon (CD3E) (AA 156-168), (Cytoplasmic Domain) antibodyCD3E 适用: 人, 大鼠, Pig, Cow, 马, 兔, 豚鼠, 小鸡 FACS, WB, IF 宿主: 小鼠 Monoclonal PC3-188A unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 25-207) antibodyCD3E 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 28-185) antibodyCD3E 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 23-207) antibodyCD3E 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (0.5-1 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Western Blotting (1-2 μg/mL for 60 minutes at RT),Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat cells. Tonsil and lymph node
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CD3 epsilon (CD3E)
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别名
- CD3E
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背景
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CD 3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex,CD3e (T-Cell Marker)
Cellular localisation: Cell Surface and Cytoplasmic -
分子量
- 20kDa
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基因ID
- 916, 3003
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UniProt
- P07766
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途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
抗原
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