CD3 epsilon 抗体 (AA 23-119)
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- 抗原 See all CD3 epsilon (CD3E) 抗体
- CD3 epsilon (CD3E)
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抗原表位
- AA 23-119
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD3 epsilon antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)
- 特异性
- Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- A recombinant human CD3e protein fragment (around aa 23-119) (exact sequence is proprietary)
- 克隆位点
- C3e-1931
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CD3E Primary Antibody
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anti-CD3 epsilon (CD3E) antibody
CD3E 适用: 人 FACS, WB, IHC 宿主: 兔 Monoclonal ARC51750 unconjugated
anti-CD3 epsilon (CD3E) (AA 23-207) antibodyCD3E 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E 适用: 人, 小鼠, 大鼠 FACS, IF, Func 宿主: 小鼠 Monoclonal RIV9 unconjugated
anti-CD3 epsilon (CD3E) (AA 156-168), (Cytoplasmic Domain) antibodyCD3E 适用: 人, Pig, 大鼠, 兔, Cow, 马, 豚鼠, 小鸡 FACS, WB, IF 宿主: 小鼠 Monoclonal PC3-188A unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 25-126) antibodyCD3E 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal CL1497 unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 25-207) antibodyCD3E 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 28-185) antibodyCD3E 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Positive Control: Jurkat cells. Tonsil or lymph node.
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- CD3 epsilon (CD3E)
- 别名
- CD3E (CD3E 产品)
- 别名
- cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody
- 分子量
- 20kDa
- 基因ID
- 916
- UniProt
- P07766
- 途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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