Cyclin A 抗体
Quick Overview for Cyclin A 抗体 (ABIN6936581)
抗原
See all Cyclin A (CCNA2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- It recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.
-
交叉反应 （详细）
- Human. Mouse. Cow. African Green Monkey. Mink (Mustela).
-
纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
-
免疫原
- Full length bovine Cyclin A protein
-
亚型
- IgG2a kappa
-
-
anti-Cyclin A2 (CCNA2) antibody
KO Validated CCNA2 适用: 人 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin A2 (CCNA2) (AA 105-233) antibodyCCNA2 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 6B4D11 unconjugated
anti-Cyclin A2 (CCNA2) (AA 1-200) antibodyCCNA2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cyclin A2 (CCNA2) (AA 51-84) antibodyCCNA2 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Cyclin A2 (CCNA2) (AA 375-431) antibodyCCNA2 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin A2 (CCNA2) antibodyCCNA2 适用: 人, 小鼠, Cow, Green Monkey, Mink IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal E67 unconjugated
anti-Cyclin A2 (CCNA2) antibodyCCNA2 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin A2 (CCNA2) (C-Term) antibodyCCNA2 适用: 人, 小鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin A2 (CCNA2) (AA 51-84), (N-Term) antibodyCCNA2 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB14617 unconjugated
anti-Cyclin A2 (CCNA2) (C-Term) antibodyCCNA2 适用: 人, 小鼠, 大鼠, Cow, 兔, 斑马鱼, 犬, 马, 豚鼠, Saccharomyces cerevisiae, 绵羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: K-562 and HeLa cells. Human tonsil or lymph node.
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
-
储存液
- Azide free
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
-
有效期
- 24 months
-
-
- Cyclin A (CCNA2) (Cyclin A2 (CCNA2))
-
别名
- CCNA2
-
背景
-
CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2,Cyclin A2 (S- & G2-phase Cyclin)
Cellular localisation: Predominantly nuclear -
分子量
- 54kDa
-
基因ID
- 890, 58974
-
UniProt
- P20248
-
途径
- PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
抗原
-
