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Cyclin A 抗体

CCNA2 适用: 人, 小鼠, Cow, Green Monkey, Mink IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal E67 unconjugated
产品编号 ABIN6940983
发货至: 中国
  • 抗原 See all Cyclin A (CCNA2) 抗体
    Cyclin A (CCNA2) (Cyclin A2 (CCNA2))
    适用
    • 88
    • 55
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    • 5
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    人, 小鼠, Cow, Green Monkey, Mink
    宿主
    • 59
    • 33
    • 1
    小鼠
    克隆类型
    • 48
    • 45
    单克隆
    标记
    • 66
    • 5
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    • 2
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    • 2
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    • 2
    • 2
    • 1
    • 1
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    This Cyclin A antibody is un-conjugated
    应用范围
    • 57
    • 30
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    • 10
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    Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
    特异性
    It recognizes a protein of 54 kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.
    纯化方法
    Purified by Protein A/G
    免疫原
    Full length bovine Cyclin A protein
    克隆位点
    E67
    亚型
    IgG2a kappa
    Top Product
    Discover our top product CCNA2 Primary Antibody
  • 应用备注

    Positive Control: K-562 and HeLa cells. Human tonsil or lymph node.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    Cyclin A (CCNA2) (Cyclin A2 (CCNA2))
    别名
    CCNA2 (CCNA2 产品)
    别名
    CCN1 antibody, CCNA antibody, AA408589 antibody, Ccn-1 antibody, Ccn1 antibody, Ccna antibody, CycA2 antibody, Cyca antibody, cb671 antibody, chunp6924 antibody, wu:fi36f03 antibody, CYCA antibody, Cyclin-A2 antibody, ccna2 antibody, ccn1 antibody, ccna antibody, ccna1 antibody, Cyclin-A antibody, cyclinA antibody, cyclin A2 antibody, cyclin A2 S homeolog antibody, cyclin A2 L homeolog antibody, CCNA2 antibody, Ccna2 antibody, ccna2 antibody, ccna2.S antibody, ccna2.L antibody
    分子量
    54kDa
    基因ID
    890
    UniProt
    P20248
    途径
    PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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