Calnexin 抗体 (AA 1-300)
Quick Overview for Calnexin 抗体 (AA 1-300) (ABIN6936576)
抗原
See all Calnexin (CANX) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 1-300
-
特异性
- It recognizes a protein of 90 kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
-
无交叉反应
- 大鼠
-
交叉反应 （详细）
- Human. Does not react with Rat.
-
纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
-
免疫原
- Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)
-
亚型
- IgG2b kappa
-
-
anti-Calnexin (CANX) (C-Term) antibody
CANX 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, Pig, 猴, Hamster, 绵羊, 小鸡, Avian, 马, 山羊, Cat, 驴 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Calnexin (CANX) (Internal Region) antibodyCANX 适用: 人, 小鼠, 大鼠 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (C-Term) antibodyCANX 适用: 犬 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (AA 263-482) antibodyCANX 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (AA 504-592) antibodyCANX 适用: 人 WB, IF, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Calnexin (CANX) (AA 1-300), (N-Term) antibodyCANX 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CANX-1541 unconjugated
anti-Calnexin (CANX) (AA 239-461) antibodyCANX 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (AA 239-461) antibodyCANX 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C8 unconjugated
anti-Calnexin (CANX) (AA 1-300), (N-Term) antibodyCANX 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CANX-1543 unconjugated
anti-Calnexin (CANX) (C-Term) antibodyCANX 适用: 犬 WB, IHC, IF, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, MCF-7 or U2OS cells. Kidney or small intestine.
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
-
储存液
- Azide free
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
-
有效期
- 24 months
-
-
- Calnexin (CANX)
-
别名
- CANX
-
背景
-
Calnexin, CANX, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88, P90,Calnexin (Endoplasmic Reticulum Marker)
Cellular localisation: Cytoplasmic -
分子量
- 90kDa
-
基因ID
- 821, 567968
-
UniProt
- P27824
-
途径
- MAPK Pathway, Thyroid Hormone Synthesis
抗原
-
