BMI1 抗体 (AA 142-326)
Quick Overview for BMI1 抗体 (AA 142-326) (ABIN6936510)
抗原
See all BMI1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 142-326
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特异性
- The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies.
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交叉反应 (详细)
- Human. Mouse.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment (around aa 142-326) of human BMI1 protein (exact sequence is proprietary)
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亚型
- IgG1 kappa
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or Jurkat cells. Colon Carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))
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别名
- BMI1
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背景
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B lymphoma Mo MLV insertion region 1 homolog, BMI1, BMI1 polycomb ring finger oncogene, FLVI2/BMI1, Oncogene BMI1, PCGF4, Polycomb complex protein BMI-1, Polycomb group ring finger 4, RING finger protein 51, RNF51,BMI1 (Oncogene and Stem Cell Marker)
Cellular localisation: Nuclear -
分子量
- 41kDa
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基因ID
- 648, 380403
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UniProt
- P35226
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途径
- Cell Division Cycle, Autophagy
抗原
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