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BMI1 抗体 (AA 142-326)

This 小鼠 单克隆 anti-BMI1 antibody (Clone BMI1-2823) specifically detects BMI1 in WB, IF 和 IHC (f). The antibody is reactive with 人 samples.
产品编号 ABIN6936510
发货至: 中国
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Quick Overview for BMI1 抗体 (AA 142-326) (ABIN6936510)

抗原

See all BMI1 抗体
BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

适用

  • 141
  • 54
  • 37
  • 10
  • 8
  • 7
  • 7
  • 6
  • 5
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1

宿主

  • 86
  • 53
  • 5
小鼠

克隆类型

  • 75
  • 69
单克隆

标记

  • 81
  • 10
  • 8
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
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  • 1
  • 1
This BMI1 antibody is un-conjugated

应用范围

  • 112
  • 76
  • 40
  • 37
  • 29
  • 23
  • 11
  • 11
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

BMI1-2823
  • 抗原表位

    • 35
    • 9
    • 7
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 142-326

    特异性

    The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies.

    交叉反应 (详细)

    Human. Mouse.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant fragment (around aa 142-326) of human BMI1 protein (exact sequence is proprietary)

    亚型

    IgG1 kappa
  • 应用备注

    Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: HeLa or Jurkat cells. Colon Carcinoma.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

    别名

    BMI1

    背景

    B lymphoma Mo MLV insertion region 1 homolog, BMI1, BMI1 polycomb ring finger oncogene, FLVI2/BMI1, Oncogene BMI1, PCGF4, Polycomb complex protein BMI-1, Polycomb group ring finger 4, RING finger protein 51, RNF51,BMI1 (Oncogene and Stem Cell Marker)
    Cellular localisation: Nuclear

    分子量

    41kDa

    基因ID

    648, 380403

    UniProt

    P35226

    途径

    Cell Division Cycle, Autophagy
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