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HSP27 抗体 (pSer78)

This anti-HSP27 antibody is a 兔 多克隆 antibody detecting HSP27 in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) 和 IHC (fro). Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN685303
发货至: 中国

Quick Overview for HSP27 抗体 (pSer78) (ABIN685303)

抗原

See all HSP27 (HSPB1) 抗体
HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This HSP27 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 抗原表位

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    pSer78

    特异性

    This phosphorylation does not exist in Mouse and Rat due to an asparagine substitution at this position.

    交叉反应

    人, 小鼠, 大鼠

    预测反应

    Dog,Cow,Horse,Rabbit

    纯化方法

    Purified by Protein A.

    免疫原

    KLH conjugated synthetic phosphopeptide derived from human HSP27 around the phosphorylation site of Ser78

    亚型

    IgG
  • 应用备注

    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 μg/μL

    缓冲液

    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    有效期

    12 months
  • 抗原

    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

    别名

    HSP27

    背景

    Synonyms: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27

    Background: Hsp27, also referred to as the Estrogen regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen responsive nature of Hsp27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors.

    基因ID

    3315

    UniProt

    P04792

    途径

    MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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