S100A8 抗体
Quick Overview for S100A8 抗体 (ABIN6757634)
抗原
See all S100A8 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes murine calgranulin A (S100 calcium-binding Protein A8) at ~10kD. In direct ELISAs and western blots, shows less than 5 % cross-reactivity with Recombinant, Murine S100A9, Recombinant, Murine S100A10 and Recombinant, Human S100B.
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纯化方法
- Immunoaffinity purified
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免疫原
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E. coli-derived, Recombinant, Murine S100A8 Calgranulin A.
Type of Immunogen: Recombinant protein -
亚型
- IgG
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), ICC, WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Western Blot, Immunocytochemistry and ELISA. Between each step, cells were washed with PBS containing 0.1 % BSA. Western Blot: 0.1-0.2 μg/mL. The detection limit is approximately 25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Direct ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect mouse S100A8. The detection limit for Recombinant, Murine S100A8 is approximately 0.2 ng/well. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 5 % trehalose, 40 % glycerol.
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
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别名
- S100A8 / MRP8
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背景
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Name/Gene ID: S100A8
Synonyms: S100A8, 60B8AG, CFAG, CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, CGLA, L1Ag, NIF, p8, Protein S100-A8, MRP8, Urinary stone protein band A, CP-10, MA387, MRP-8 -
基因ID
- 6279
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UniProt
- P05109
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途径
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
抗原
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