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FZD4 抗体 (Extracellular Domain)

The 大鼠 单克隆 anti-FZD4 antibody (ABIN6757418) specifically detects FZD4 in WB, FACS 和 ICC. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN6757418
发货至: 中国
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Quick Overview for FZD4 抗体 (Extracellular Domain) (ABIN6757418)

抗原

See all FZD4 抗体
FZD4 (Frizzled Family Receptor 4 (FZD4))

适用

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人, 小鼠

宿主

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  • 5
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大鼠

克隆类型

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  • 14
  • 2
单克隆

标记

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This FZD4 antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
  • 抗原表位

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    Extracellular Domain

    特异性

    Recognizes mouse Frizzled-4. Shows no cross-reactivity with rmFrizzled-1, -2, 3, -6, -7 -8 or rhFrizzled-5. Species cross-reactivity: Human. Detects Frizzled-4 by Flow Cytometry and Immunohistochemistry on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Also recognizes human Frizzled-4 by Flow Cytometry and Immunohistochemistry on HEK 293 cells.

    纯化方法

    Protein G purified

    免疫原

    NS0-derived rmFrizzled-4 extracellular domain.

    亚型

    IgG2a
  • 应用备注

    Approved: Flo (100 μg/mL), ICC (10 μg/mL), WB (1 - 2 μg/mL)

    Usage: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Flow Cytometry: 100 μg/mL. Add 10 μL to 1-2.5x10^5 cells. Total reaction volume should not exceeding 200 μL. Immunocytochemistry: 10 μg/mL in 4 % paraformaldehyde-fixed cells. Western Blot:1-2 μg/mL. The detection limit is ~5 ng/lane under non-reducing and reducing conditions.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Liquid

    溶解方式

    Sterile PBS

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.2, 5 % trehalose.

    注意事项

    avoid freeze thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • 抗原

    FZD4 (Frizzled Family Receptor 4 (FZD4))

    别名

    FZD4 / Frizzled 4

    背景

    Name/Gene ID: FZD4
    Subfamily: Frizzled
    Family: GPCR

    Synonyms: FZD4, CD344, CD344 antigen, EVR1, Frizzled family receptor 4, Frizzled-4, Fz-4, Fz4, HFz4, Exudative vitreoretinopathy 1, FEVR, FZD4S, FzE4, Frizzled 4, Frizzled homolog 4, WNT receptor frizzled-4

    基因ID

    8322

    UniProt

    Q9ULV1

    途径

    WNT signaling, Hormone Transport, Sensory Perception of Sound
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