FZD4 抗体 (N-Term)
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- 抗原 See all FZD4 抗体
- FZD4 (Frizzled Family Receptor 4 (FZD4))
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抗原表位
- N-Term
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适用
- 人, 大鼠, Cow, 犬, 猴, Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This FZD4 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human FZD4 / Frizzled 4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Rat, Dolphin, Hamster, Elephant, Panda, Dog, Bovine (100%) Mouse, Horse, Pig (94%) Rabbit (89%) Lizard (83%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 18 amino acid peptide from N-terminal extracellular domain of human FZD4 / Frizzled 4. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Rat, Dolphin, Hamster, Elephant, Panda, Dog, Bovine (100%), Mouse, Horse, Pig (94%), Rabbit (89%), Lizard (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product FZD4 Primary Antibody
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anti-Frizzled Family Receptor 4 (FZD4) (AA 37-222) antibody
FZD4 适用: 人 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 2F3A7 unconjugated
anti-Frizzled Family Receptor 4 (FZD4) (Middle Region) antibodyFZD4 适用: 人, 小鼠, 大鼠, Cow, 马, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 4 (FZD4) (AA 11-60) antibodyFZD4 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 4 (FZD4) (AA 37-222) antibodyFZD4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 4 (FZD4) (AA 100-200) antibody (Atto 594)FZD4 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Atto 594
anti-Frizzled Family Receptor 4 (FZD4) (AA 100-200) antibody (Atto 488)FZD4 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Atto 488
anti-Frizzled Family Receptor 4 (FZD4) (AA 100-200) antibody (Atto 390)FZD4 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Atto 390
anti-Frizzled Family Receptor 4 (FZD4) (AA 107-206) antibodyFZD4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3G7 unconjugated
anti-Frizzled Family Receptor 4 (FZD4) (AA 100-200) antibody (FITC)FZD4 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal FITC
anti-Frizzled Family Receptor 4 (FZD4) (AA 100-200) antibody (Alkaline Phosphatase (AP))FZD4 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
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- 应用备注
- Approved: IHC, IHC-P (30 - 60 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- FZD4 (Frizzled Family Receptor 4 (FZD4))
- 别名
- FZD4 / Frizzled 4 (FZD4 产品)
- 别名
- CG4626 antibody, DFz4 antibody, Dfz4 antibody, Dm Fz4 antibody, Dmel\\CG4626 antibody, Fz4 antibody, anon-WO0170980.10 antibody, anon-WO0170980.11 antibody, fz1 antibody, zg01 antibody, CD344 antibody, EVR1 antibody, FEVR antibody, FZD4S antibody, Fz-4 antibody, FzE4 antibody, GPCR antibody, hFz4 antibody, frizzled4 antibody, fz4 antibody, FZ-4 antibody, frizzled 4 antibody, frizzled class receptor 4 antibody, frizzled-4 antibody, frizzled class receptor 4 S homeolog antibody, fz4 antibody, fzd4 antibody, Tsp_10376 antibody, FZD4 antibody, Fzd4 antibody, fzd4.S antibody
- 背景
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Name/Gene ID: FZD4
Subfamily: Frizzled
Family: GPCR
Synonyms: FZD4, CD344, CD344 antigen, EVR1, Frizzled family receptor 4, Frizzled-4, Fz-4, Fz4, HFz4, Exudative vitreoretinopathy 1, FEVR, FZD4S, FzE4, Frizzled 4, Frizzled homolog 4, WNT receptor frizzled-4 - 基因ID
- 8322
- 途径
- WNT signaling, Hormone Transport, Sensory Perception of Sound
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