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PODXL 抗体 (AA 301-350)

PODXL 适用: 人, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN6741605
发货至: 中国
  • 抗原 See all PODXL 抗体
    PODXL (Podocalyxin-Like (PODXL))
    抗原表位
    • 16
    • 13
    • 12
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 301-350
    适用
    • 83
    • 23
    • 14
    • 8
    • 1
    人, 猴
    宿主
    • 53
    • 40
    • 1
    克隆类型
    • 53
    • 41
    多克隆
    标记
    • 46
    • 8
    • 8
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PODXL antibody is un-conjugated
    应用范围
    • 68
    • 32
    • 30
    • 27
    • 22
    • 21
    • 9
    • 9
    • 7
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human PODXL
    预测反应
    Percent identity by BLAST analysis: Human (100%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic peptide located between aa301-350 of human PODXL (A6NHX8, NP_001018121). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey (100%), Gorilla (92%).

    Type of Immunogen: Synthetic peptide
    亚型
    IgG
    Top Product
    Discover our top product PODXL Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (5 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Distilled Water.
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS with 2 % sucrose
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原
    PODXL (Podocalyxin-Like (PODXL))
    别名
    PODXL / Podocalyxin (PODXL 产品)
    别名
    Gp200 antibody, PC antibody, PCLP antibody, PCLP-1 antibody, PCB antibody, AW121214 antibody, Ly102 antibody, Pclp1 antibody, podocalyxin antibody, MEP21 antibody, PODXL antibody, Gp135 antibody, PCLP1 antibody, cPCLP1 antibody, podxl antibody, fc18d02 antibody, wu:fc18d02 antibody, Pc antibody, Pcb antibody, Podxl antibody, podocalyxin like antibody, pyruvate carboxylase antibody, podocalyxin-like antibody, PODXL antibody, PC antibody, Podxl antibody, podxl antibody, Pcx antibody
    背景
    Name/Gene ID: PODXL

    Synonyms: PODXL, GCTM-2 antigen, gp200, PCLP, PCLP-1, PCLP1, Podocalyxin-like protein 1, Podocalyxin, Podocalyxin-like, TRA-1-60, TRA-1-81
    基因ID
    5420
    NCBI登录号
    NP_001018121
    UniProt
    O00592
    途径
    Tube Formation
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