Recognizes native and denatured-reduced forms of synthetic poly-histidine or poly-histidine-tagged fusion proteins. Recognizes fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.
Usage: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Western Blot: 1:10000. ELISA: 1:10000-1:50000 using 50-100 ng control antigen/well. Immunohistochemistry: 1:200-1:2000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
限制
仅限研究用
状态
Liquid
浓度
Lot specific
缓冲液
0.05 % merthiolate
储存液
Thimerosal (Merthiolate)
注意事项
This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
avoid freeze thaw cycles.
储存条件
4 °C,-20 °C
储存方法
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.