FASL 抗体 (C-Term)
Quick Overview for FASL 抗体 (C-Term) (ABIN6735258)
抗原
See all FASL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- Sequence is 94 % conserved in mouse. It is poorly conserved in human FASL. 92 % homologous with rat and mouse FASL. Based upon the sequence homology data, anti human FASL should cross-react with the mouse/rat FASL.
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纯化方法
- Immunoaffinity purified
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免疫原
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13-aa peptide from human FASL near the C terminus.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), WB (1:1000 - 1:5000)
Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000 (ECL). We recommend the use of 0.5-1 % milk in diluents used for primary antibody and secondary antibody-enzyme conjugates to suppress background. Rat, mouse and human FASL have 278, 279, and 281aa residues, respectively (Calculated MW of ~32kD). Western blot of the oocyte has detected a specific 31-kD band. A 40kD FASL has been detected in mouse T cells. An alternate detecting reagent, mFAS-Fc also detected a cell surface protein of about 40kD. ELISA: 0.5-1 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 100 mM Tris, pH 7.5, 0.2 % BSA, 40 % glycerol.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
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别名
- FASLG / FASL / Fas Ligand
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背景
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Name/Gene ID: FASLG
Family: TNF
Synonyms: FASLG, ALPS1B, Apoptosis antigen ligand, APTL, CD95L, CD95 ligand, CD178 antigen, CD95-L, Fas antigen ligand, Fas ligand, FASL, TNFSF6, APT1LG1, CD178 -
基因ID
- 356
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UniProt
- P48023
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途径
- Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
抗原
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