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BRCA2 抗体 (AA 21-130)

Name: BRCA2 抗体 (AA 21-130)
SKU: ABIN673434
Availability: OutOfStock
Rating: 5 (1 reviews)

This anti-BRCA2 antibody is a 兔多克隆 antibody detecting BRCA2 in WB, ELISA, IHC (p), IHC (fro), IF (cc) 和 IF (p). Suitable for 人. Independently validated for use in Western Blotting.

Western Blotting validation image for anti-Breast Cancer 2, Early Onset (BRCA2) (AA 21-130) antibody (ABIN673434) (BRCA2 抗体 (AA 21-130))

Image provided by the Independent Validation Program badge 29807. (BRCA2 抗体 (AA 21-130))

Formalin-fixed and paraffin embedded human breast carcinoma labeled Anti-BRCA2 Polyclonal Antibody, Unconjugated (ABIN673434) at 1:200, followed by conjugation to the secondary antibody and DAB staining (BRCA2 抗体 (AA 21-130))

IHC

3 images

(1 validation)

产品编号 ABIN673434

发货至: 中国

Datasheet as PDF

Quick Overview for BRCA2 抗体 (AA 21-130) (ABIN673434)

抗原

See all BRCA2 抗体

BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

适用

人

宿主

兔

克隆类型

多克隆

标记

This BRCA2 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

抗原表位
12

6

5

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 21-130

交叉反应

人

预测反应

Mouse,Rat,Dog,Cow,Horse,Chicken,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human BRCA2

亚型

IgG
anti-Breast Cancer 2, Early Onset (BRCA2) antibody
BRCA2 适用: 人, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (AA 2990-3232) antibody
BRCA2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (AA 3319-3418) antibody
BRCA2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D12 unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (AA 1-300) antibody
BRCA2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (AA 2913-3151) antibody
BRCA2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (AA 2308-2537) antibody
BRCA2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (AA 2737-2874) antibody
BRCA2 适用: 斑马鱼 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (pSer3291) antibody
BRCA2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (AA 251-495) antibody
BRCA2 适用: 人 WB 宿主: 小鼠 Monoclonal 1643CT739-91-87 unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (N-Term) antibody
BRCA2 适用: 人, 大鼠, 猴 WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #029807 (Western Blotting)

by

Alamo Laboratories Inc

No.

#029807

日期

2014.08.26

抗原

Lot Number

101020

Method validated

Western Blotting

Positive Control

MCF7 cells

Negative Control

[CAPAN1 cells] (http://www.pnas.org/content/98/15/8644.full.pdf)

Notes

A strong band was observed in the positive control sample at the correct molecular weight. No bands were observed in the negative control sample.

Validation Images

Figure 1: Western Blot for BRCA2. Arrowhead indicates the expected molecular weight of ~384 kDa.
Full Methods
Primary Antibody

Antigen: Breast Cancer 2, Early Onset (BRCA2)
Catalog number: ABIN673434
Lot number: 101020
Antibody Dilution: 1:200

Secondary Antibody

Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
Lot number: L170-6515
Antibody Dilution: 1:10,000

Full Protocol

1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
2. 15 μl of heated culture-media were loaded and resolved on 8-16% SDS-polyacrylamide gel.
3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
6. The membrane was rinsed with TBST once.
7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 10 hours at 4°C.
8. The membrane was rinsed in TBST thrice for 5 minutes each.
9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
11. The membrane was rinsed in TBS twice for 30 seconds each.
12. Signals were detected with ECL-2 Substrate. The blot was scanned for 5 minutes.
13. The membrane was rinsed three times TBST.
14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
15. The membrane was washed in TBST 2 times for 10 minutes each.
16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.

Experimental Notes

- No experimental challenges noted.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

别名

BRCA2

背景

Synonyms: FAD, FACD, FAD1, GLM3, BRCC2, FANCD, PNCA2, FANCD1, BROVCA2, Breast cancer type 2 susceptibility protein, Fanconi anemia group D1 protein, BRCA2
Background: Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks, together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and DSS1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:24896180).

基因ID

675

UniProt

P51587

途径

DNA Damage Repair, M Phase, Maintenance of Protein Location