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CDKN1B 抗体 (AA 150-198)

Name: CDKN1B 抗体 (AA 150-198)
SKU: ABIN672621
Availability: OutOfStock

Cited in 5+ publications. This 兔多克隆 anti-CDKN1B antibody specifically detects CDKN1B in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) 和 IHC (fro). The antibody is reactive with 人, 小鼠和大鼠 samples.

Dnmt3a3 overexpression decreases fat accumulation in differentiating preadipocytes. (CDKN1B 抗体 (AA 150-198))

Formalin-fixed and paraffin embedded human ovary carcinoma labeled Anti-CDKN1B/P27kip1 Polyclonal Antibody, Unconjugated (ABIN672621) at 1:200, followed by conjugation to the secondary antibody and DAB staining (CDKN1B 抗体 (AA 150-198))

IHC

Mouse lung lysates probed with CDKN1B Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C. (CDKN1B 抗体 (AA 150-198))

MCF-7 cells were stained with CDKN1B Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. (CDKN1B 抗体 (AA 150-198))

IF (cc)

Formalin-fixed and paraffin embedded human ovary carcinoma labeled Anti-CDKN1B/P27kip1 Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (CDKN1B 抗体 (AA 150-198))

IHC (p)

RSC96 probed with CDKN1B Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange). (CDKN1B 抗体 (AA 150-198))

FACS

Expression of Dnmt3a spliced variants in adipose tissues and during the time-course analysis of preadipocyte differentiation. (CDKN1B 抗体 (AA 150-198))

7 images

(5 references)

产品编号 ABIN672621

发货至: 中国

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中国
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Quick Overview for CDKN1B 抗体 (AA 150-198) (ABIN672621)

抗原

See all CDKN1B 抗体

CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This CDKN1B antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

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AA 150-198

交叉反应

小鸡, 人, 小鼠, 大鼠

预测反应

Dog,Sheep,Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human CDKN1B

亚型

IgG
anti-P27 antibody
CDKN1B 适用: 人, 小鼠, 大鼠, 犬 WB 宿主: 小鼠 Monoclonal DCS72 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibody
CDKN1B 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 4B4-E6 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
KO Validated CDKN1B 适用: 人 WB, IHC, IF, IHC (p), IP, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
CDKN1B 适用: 人 IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal SX53G8 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
KD Validated CDKN1B 适用: 人 WB, FACS, ICC 宿主: 兔 Monoclonal 23GB1825 unconjugated Recombinant Antibody

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
CDKN1B 适用: 人, 小鼠, 大鼠, 猴 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal DCS-72-F6-KIP1-769 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibody
CDKN1B 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
CDKN1B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 1G2 unconjugated Recombinant Antibody

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr187) antibody
CDKN1B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibody
CDKN1B 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

应用备注

WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Abdalla, Li, Nie: "A Novel DNA Methyltransferase Dnmt3a3 Splice Variant Represses Preadipocyte Proliferation and Differentiation." in: Frontiers in genetics, Vol. 11, pp. 115, (2020) (PubMed).

Guo, Xu, Ren, Zhang, Zhang, Duan, Zhou, Sun: "Endosulfan induces apoptosis by activating the negative regulation pathway of cell cycle and death receptor pathway in spermatogenic cells." in: Toxicology research, Vol. 6, Issue 2, pp. 223-231, (2017) (PubMed).

Akizuki, Shimobaba, Matsunaga, Endo, Ikari: "Claudin-5, -7, and -18 suppress proliferation mediated by inhibition of phosphorylation of Akt in human lung squamous cell carcinoma." in: Biochimica et biophysica acta, Vol. 1864, Issue 2, pp. 293-302, (2016) (PubMed).

Gao, Tang, Ding, Wang, Qi, Wu, Guo: "Protein-Binding Function of RNA-Dependent Protein Kinase Promotes Proliferation through TRAF2/RIP1/NF-?B/c-Myc Pathway in Pancreatic ? cells." in: Molecular medicine (Cambridge, Mass.), Vol. 21, Issue 1, pp. 154-66, (2015) (PubMed).

Sun, Yang, Luo, Wang, Chen, Zhang, Wang, Li: "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." in: Theriogenology, (2014) (PubMed).
抗原

CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

别名

CDKN1B

背景

Synonyms: KIP1, MEN4, CDKN4, MEN1B, P27KIP1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, CDKN1B
Background: Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

基因ID

1027

UniProt

P46527

途径

Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy