GFP 抗体 (DyLight 680)
(1 reference)
Quick Overview for GFP 抗体 (DyLight 680) (ABIN6720707)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-144-215
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Supplier
- Rockland
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原理
- GFP Antibody Dylight™ 680 Conjugated
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特异性
- Wt, rGFP, eGFP
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交叉反应 （详细）
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Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). -
纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
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亚型
- IgG
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Labeling Ratio
- 2.8
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anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IHC, IP, ICC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FM, FACS, DB 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FM, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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FLISA_Dilution: >1:20,000
ELISA_Dilution: 1:20,000 - 1:40,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: 1:10,000 - 1:25,000
Other: User Optimized
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说明
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Suggested Applications: IF
The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Shigella promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, Issue 27, pp. 13582-13591, (2020) (PubMed).
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: "Shigella promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, Issue 27, pp. 13582-13591, (2020) (PubMed).
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- GFP (Green Fluorescent Protein (GFP))
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别名
- GFP
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背景
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Synonyms: goat anti-GFP Antibody DyLight™ 680 Conjugation, DyLight™ 680 conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms such as the LICOR Odyssey Imaging system. DyLight conjugated antibodies are effective alternatives to Alexa 680 and IR Dye 680 conjugates.
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UniProt
- P42212
抗原
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