GFP 抗体 (DyLight 488)
(1 reference)
Quick Overview for GFP 抗体 (DyLight 488) (ABIN6720700)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
Supplier Product No.
- 200-341-215
-
Supplier
- Rockland
-
原理
- GFP Antibody Dylight™ 488 Conjugated
-
特异性
- Wt, rGFP, eGFP
-
交叉反应 （详细）
- Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum.
-
纯化方法
- GFP Dylight™ 488 Conjugated Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography.
-
免疫原
- BALB/c mice were immunized with a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. A hybridoma was produced by the fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology.
-
亚型
- IgG1 kappa
-
Labeling Ratio
- 3.2
-
-
anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IHC, IP, ICC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FM, FACS, DB 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FM, FACS 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: 1:10,000 - 1:25,000
Other: User Optimized
-
说明
-
Suggested Applications: IF
Anti-GFP Dylight™ 488 Conjugated has been tested by ELISA and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
-
: "Application of Aptamers Improves CRISPR-Based Live Imaging of Plant Telomeres." in: Frontiers in plant science, Vol. 11, pp. 1254, (2020) (PubMed).
-
: "Application of Aptamers Improves CRISPR-Based Live Imaging of Plant Telomeres." in: Frontiers in plant science, Vol. 11, pp. 1254, (2020) (PubMed).
-
- GFP (Green Fluorescent Protein (GFP))
-
别名
- GFP
-
背景
-
Synonyms: mouse anti-GFP Antibody Dylight™ 488 Conjugation, Dylight™ 488 Conjugated mouse anti-GFP Antibody, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Background: GFP monoclonal antibody is Dylight™ 488 Conjugated. Labeled GFP Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. DyLight™ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods.
-
UniProt
- P42212
抗原
-
