Fluorescein 抗体 (DyLight 405)
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北京 101111
Quick Overview for Fluorescein 抗体 (DyLight 405) (ABIN6720644)
抗原
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-146-096
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Supplier
- Rockland
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原理
- Fluorescein Antibody Dylight™ 405 Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.
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纯化方法
- Fluorescein Antibody Dylight™ 405 Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- Fluorescein conjugated to Goat IgG
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亚型
- IgG
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Labeling Ratio
- 4.1
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anti-Fluorescein antibody
WB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated
anti-Fluorescein antibodyWB, ELISA, IHC, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Fluorescein antibodyWB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated
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应用备注
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FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000-1:25,000
Other: User Optimized
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说明
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Anti-Fluorescein Antibody Dylight™ 405 Conjugated has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Fluorescein
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物质类
- Chemical
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背景
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Synonyms: goat Anti-FITC antibody DyLight™ 405 conjugation, DyLight™ 405 conjugated goat anti-fluorescein antibody, DyLight™ 405 conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody DyLight™ 405 conjugation
Background: Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Dylight™ antibody and reagent conjugates are specifically designed for protein detection methods that cover much of the visible spectrum and into the near-infrared region to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. Dylight™conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging.
抗原
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