(2 references)-
- 抗原
- Fluorescein
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)
- 产品特性
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Synonyms: mouse Anti-FITC antibody, mouse anti-fluorescein antibody
Background: Anti-Fluorescein is designed for immunofluorescence microscopy, in situ hybridization, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- 纯化方法
- Fluorescein Antibody was prepared from mouse ascites by Protein A chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Fluorescein conjugated IgG and Fluorescein conjugated Bovine Serum Albumin.
- 免疫原
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Immunogen: Fluorescein conjugated to Mouse IgG
Immunogen Type: Other
- 克隆位点
- 8B9-C6-D3
- 亚型
- IgG2a
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anti-Fluorescein antibody
FACS, IM 宿主: 小鼠 Monoclonal unconjugated
anti-Fluorescein antibodyFACS, IM 宿主: 山羊 Polyclonal unconjugated
anti-Fluorescein antibody (HRP)WB, ELISA, IHC, DB, IEM 宿主: 山羊 Polyclonal HRP
anti-Fluorescein antibody (Biotin)WB, ELISA, IHC, IM 宿主: 山羊 Polyclonal Biotin
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- 应用备注
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Flow Cytometry Dilution: 1-2 μg/mL
Immunohistochemistry Dilution: 1:250 - 1:1,500
Application Note: Anti-Fluorescein Antibody is suitable for immunomicroscopy,in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:30,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 12 months
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: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).
: "Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains." in: PLoS ONE, Vol. 9, Issue 3, pp. e93054, (2015) (PubMed).
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: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).
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- 抗原
- Fluorescein
- 物质类
- Chemical
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