(2 references)-
- 抗原
- Fluorescein
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- ELISA
- 特异性
- This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against Fluorescein conjugated Rabbit IgG and Fluorescein conjugated KLH.
- 产品特性
- This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- 过滤
- Sterile filtered
- 免疫原
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Fluorescein conjugated Bovine Serum Albumin (BSA)
Immunogen Type: NativeProtein
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anti-Fluorescein antibody
FACS, IM 宿主: 小鼠 Monoclonal unconjugated
anti-Fluorescein antibodyFACS, IM 宿主: 山羊 Polyclonal unconjugated
anti-Fluorescein antibody (HRP)WB, ELISA, IHC, DB, IEM 宿主: 山羊 Polyclonal HRP
anti-Fluorescein antibody (Biotin)WB, ELISA, IHC, IM 宿主: 山羊 Polyclonal Biotin
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- 应用备注
- This product has been assayed against 1.0 ug of Fluorescein conjugated Rabbit IgG in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] Donkey) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100,000 to 1:500,000 of the reconstitution concentration is suggested for this product.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
- 浓度
- 90 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
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: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).
: "Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains." in: PLoS ONE, Vol. 9, Issue 3, pp. e93054, (2015) (PubMed).
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: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).
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- 抗原
- Fluorescein
- 物质类
- Chemical
- 背景
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This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Synonyms: FITC
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