His Tag 抗体 (DyLight 649)
(5 references)
Quick Overview for His Tag 抗体 (DyLight 649) (ABIN6720637)
抗原
See all His Tag 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-343-382
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Supplier
- Rockland
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原理
- 6X His Tag Antibody DyLight™ 649
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交叉反应 （详细）
- This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.
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纯化方法
- This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays.
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免疫原
- This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
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亚型
- IgG1 kappa
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Labeling Ratio
- 2.5
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anti-His Tag antibody
WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated
anti-His Tag antibody (Cy3)WB, ELISA, FACS, DB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 Cy3
anti-His Tag antibody (DyLight 800)WB, ELISA, DB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 800
anti-His Tag antibody (HRP)WB, ELISA, IHC 宿主: 小鼠 Monoclonal 33D10-D2-G8 HRP
anti-His Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 3G5H8 unconjugated
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应用备注
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FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000-1:25,000
Other: FLUORESCENT POLARIZATION: 2 - 20 nM
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说明
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Suggested Applications: Biochemical Assay, Microarray
Anti-6X HIS tagged Antibody conjugated to DyLight™649 has been tested by ELISA, dot blot, and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. Anti-6X HIS Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Dual Bcl-XL /Bcl-2 inhibitors discovered from DNA-encoded libraries using a fragment pairing strategy." in: Bioorganic & medicinal chemistry, Vol. 44, pp. 116282, (2021) (PubMed).
: "An engineered ligand trap inhibits leukemia inhibitory factor as pancreatic cancer treatment strategy." in: Communications biology, Vol. 4, Issue 1, pp. 452, (2021) (PubMed).
: "An engineered antibody binds a distinct epitope and is a potent inhibitor of murine and human VISTA." in: Scientific reports, Vol. 10, Issue 1, pp. 15171, (2020) (PubMed).
: "Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies." in: The Journal of clinical investigation, Vol. 127, Issue 1, pp. 183-198, (2017) (PubMed).
: "An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis." in: Nature chemical biology, Vol. 10, Issue 11, pp. 977-83, (2014) (PubMed).
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: "Dual Bcl-XL /Bcl-2 inhibitors discovered from DNA-encoded libraries using a fragment pairing strategy." in: Bioorganic & medicinal chemistry, Vol. 44, pp. 116282, (2021) (PubMed).
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- His Tag
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背景
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Synonyms: mouse anti-6X His Tag DyLight™ 649 conjugated Antibody, DyLight™ 649 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
Background: Anti 6X HIS tagged Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. Anti-6X HIS Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
抗原
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