Calpain 2 抗体 (AA 500-700)
Quick Overview for Calpain 2 抗体 (AA 500-700) (ABIN6719643)
抗原
See all Calpain 2 (CAPN2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 500-700
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原理
- Anti-Calpain 2 CAPN2 Antibody Picoband® (monoclonal, 8I6)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Calpain 2 CAPN2 Antibody Picoband® (monoclonal, 8I6) (ABIN6719643). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human Calpain 2 recombinant protein (Position: R500-L700). Human Calpain 2 shares 93.5% and 92.5% amino acid (aa) sequence identity with mouse and rat Calpain 2, respectively.
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亚型
- IgG2a
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anti-Calpain 2 (CAPN2) (AA 20-700), (Catalytic Subunit) antibody
CAPN2 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Calpain 2 (CAPN2) (AA 45-514) antibodyCAPN2 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calpain 2 (CAPN2) (AA 1-400) antibodyCAPN2 适用: 人 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal 1381CT669-7-66-71 unconjugated
anti-Calpain 2 (CAPN2) (AA 350-680) antibodyCAPN2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Calpain 2 (CAPN2) (AA 489-700) antibodyCAPN2 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2E2F2 unconjugated
anti-Calpain 2 (CAPN2) (AA 500-700) antibodyCAPN2 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Calpain 2 (CAPN2) antibodyCAPN2 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal 1C1 unconjugated
anti-Calpain 2 (CAPN2) (AA 301-335) antibodyCAPN2 适用: 人 WB 宿主: 兔 Polyclonal RB52877 unconjugated
anti-Calpain 2 (CAPN2) (AA 1-700) antibodyCAPN2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Calpain 2 (CAPN2) antibodyCAPN2 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
1. Hata A, Ohno S, Akita Y, Suzuki K (May 1989). "Tandemly reiterated negative enhancer-like elements regulate transcription of a human gene for the large subunit of calcium-dependent protease". J. Biol. Chem. 264 (11): 6404-11. 2. Imajoh S, Aoki K, Ohno S, Emori Y, Kawasaki H, Sugihara H, Suzuki K (May 1989). "Molecular cloning of the cDNA for the large subunit of the high-Ca2+-requiring form of human Ca2+-activated neutral protease". Biochemistry. 27 (21): 8122-8. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Calpain 2 (CAPN2)
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别名
- CAPN2
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背景
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Synonyms: Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CAPN2, CANPL2
Tissue Specificity: Ubiquitous.
Background: Calpain-2 catalytic subunit is a protein that in humans is encoded by the CAPN2 gene. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported.
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分子量
- 80 kDa
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基因ID
- 824
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UniProt
- P17655
抗原
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