H2AFX 抗体 (AA 2-121)
Quick Overview for H2AFX 抗体 (AA 2-121) (ABIN6719574)
抗原
See all H2AFX 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-121
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原理
- Anti-Histone H2A.X/H2AFX Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Histone H2A.X/H2AFX Antibody Picoband® (ABIN6719574). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Histone H2A.X/H2AFX recombinant protein (Position: S2-T121).
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亚型
- IgG
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anti-H2A Histone Family, Member X (H2AFX) (AA 115-143), (C-Term) antibody
H2AFX 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 938CT5-1-1 unconjugated
anti-H2A Histone Family, Member X (H2AFX) (pSer140) antibodyH2AFX 适用: 人 WB, ELISA, IHC (p), ICC, FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member X (H2AFX) (ubLys119) antibodyH2AFX 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member X (H2AFX) antibodyH2AFX 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 8C5 unconjugated
anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibodyH2AFX 适用: 人 WB, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibodyH2AFX 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibodyH2AFX 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member X (H2AFX) (C-Term) antibodyH2AFX 适用: 人 WB, IF, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member X (H2AFX) (pTyr143) antibodyH2AFX 适用: 人, 小鼠, 大鼠 ELISA, IHC (p), ICC, FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibodyH2AFX 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Monoclonal 1C14 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM (1998). "DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139". J. Biol. Chem. 273 (10): 5858-68. 2. Tanaka T, Halicka HD, Huang X, Traganos F, Darzynkiewicz Z. (2006) Constitutive histone H2AX phosphorylation and ATM activation, the reporters of DNA damage by endogenous oxidants. Cell Cycle. 5:1940-5. Epub 2006 Sep 1. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- H2AFX (H2A Histone Family, Member X (H2AFX))
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别名
- H2AFX
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背景
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Synonyms: Histone H2AX, H2a/x, Histone H2A.X, H2AFX, H2AX
Tissue Specificity: Expressed in the cerebellum and spinal motor neurons.
Background: H2A histone family member X (usually abbreviated asH2AX) is a type of histone protein from the H2A family encoded by the H2AFX gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
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分子量
- 15 kDa
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基因ID
- 3014
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UniProt
- P16104
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途径
- Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
抗原
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