SMAD2 抗体 (AA 83-264)
Quick Overview for SMAD2 抗体 (AA 83-264) (ABIN6719361)
抗原
See all SMAD2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 83-264
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原理
- Anti-SMAD2 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-SMAD2 Antibody (ABIN6719361). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human SMAD2 recombinant protein (Position: E83-Q264).
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亚型
- IgG
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anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibody
SMAD2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3G9 unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 2-467) antibodySMAD2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pSer423), (pSer425), (pSer465), (pSer467) antibodySMAD2 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (C-Term) antibodySMAD2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibodySMAD2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4D10 unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pSer467) antibodySMAD2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pThr183), (pThr221) antibodySMAD2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (N-Term) antibodySMAD2 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibodySMAD2 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2D7 unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 94-112), (N-Term) antibodySMAD2 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Eppert, K., Scherer, S. W., Ozcelik, H., Pirone, R., Hoodless, P., Kim, H., Tsui, L.-C., Bapat, B., Gallinger, S., Andrulis, I. L., Thomsen, G. H., Wrana, J. L., Attisano, L.MADR2 maps to 18q21 and encodes a TGF-beta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma.Cell 86: 543-552, 1996. 2. Nakao, A., Roijer, E., Imamura, T., Souchelnytskyi, S., Stenman, G., Heldin, C.-H., ten Dijke, P.Identification of Smad2, a human Mad-related protein in the transforming growth factor-beta signaling pathway.J. Biol. Chem. 272: 2896-2900, 1997. 3. Riggins, G. J., Thiagalingam, S., Rozenblum, E., Weinstein, C. L., Kern, S. E., Hamilton, S. R., Willson, J. K. V., Markowitz, S. D., Kinzler, K. W., Vogelstein, B.Mad-related genes in the human.Nature Genet. 13: 347-349, 1996. -
说明
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
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别名
- SMAD2
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背景
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Synonyms: Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, hSMAD2, Smad2, SMAD2, MADH2, MADR2
Tissue Specificity: Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
Background: Smad2 (Mothers against decapentaplegic homolog 2), also known as MADR2, MADH2, SMAD family member 2 or SMAD2, is a protein that in humans is encoded by the SMAD2 gene. MAD homolog 2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. Eppert et al. mapped the MADR2 gene close to DPC4 at 18q21, a region which is frequently deleted in colorectal cancers. Riggins et al. mapped the human MADH2 gene to 18q21. Nakao et al. refined the localization of the SMAD2 gene to 18q21.1, approximately 3 Mb proximal to DPC4, by fluorescence in situ hybridization. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.
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分子量
- 60 kDa
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基因ID
- 4087
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UniProt
- Q15796
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途径
- Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
抗原
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