LAMP2 抗体
Quick Overview for LAMP2 抗体 (ABIN6657933)
抗原
See all LAMP2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-501-g10
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Supplier
- Rockland
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原理
- Lamp2 Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with LAMP2 from mouse and rabbit based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-LAMP2 Antibody was purified by Protein G chromatography.
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过滤
- Sterile filtered
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免疫原
- LAMP2 Antibody was produced in rat by repeated immunizations with purified preparation of mouse liver lysosomal membranes.
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亚型
- IgG1
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anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (AA 191-362) antibody
LAMP2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (AA 29-168) antibodyLAMP2 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 6A10H10 unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (AA 29-168) antibodyLAMP2 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 8E2F2 unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (AA 301-411) antibodyLAMP2 适用: 人, 小鼠, 大鼠, Pig WB, FACS, ELISA, IHC (fro), IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (AA 193-375) antibodyLAMP2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) antibodyLAMP2 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) antibodyLAMP2 适用: 人 WB, FACS, ICC, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal H4B4 unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) antibodyKO Validated LAMP2 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (AA 54-268) antibodyLAMP2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (AA 30-127) antibodyLAMP2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G10 unconjugated
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应用备注
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Immunoprecipitation_Dilution: User Optimized
IF_Microscopy_Dilution: 1:500- 1:1000
Western_Blot_Dilution: 1:1000
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说明
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Anti-LAMP2 Antibody is tested for use in WB, IF microscopy, and IP. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))
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别名
- LAMP2
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背景
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Synonyms: CD107b, Igp110, Igp2, Lamp2C, LampB, MAC3, Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, Lysosomal membrane glycoprotein type B, LGP-B
Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology. They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane. Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxylterminal tyrosine based motif (YXXI). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome. This stimulation has also been shown to have an associated release of histamine, leukotriene C and prostaglandin D, which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells. They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation. Anti-LAMP2 antibody is ideal for investigators involved in cytokines, growth factors and cancer research, as well as, Chaperones and Organelle Markers research.
Gene Name: LAMP2
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基因ID
- 16784
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NCBI登录号
- NP_001017959
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UniProt
- P17047
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途径
- Autophagy
抗原
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