CD3 epsilon 抗体 (FITC)
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北京 101111
Quick Overview for CD3 epsilon 抗体 (FITC) (ABIN6657857)
抗原
See all CD3 epsilon (CD3E) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-b02-m69
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Supplier
- Rockland
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原理
- CD3e Fluorescein Antibody
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交叉反应 （详细）
- Reactivity is observed against mouse CD3.
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纯化方法
- Fluorescein conjugated CD3 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against mouse CD3.
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过滤
- Sterile filtered
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免疫原
- Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with H-2Kb specific cytotoxic T lymphocyte clone BM10-37.
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亚型
- IgG
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Labeling Ratio
- 2-8
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anti-CD3 epsilon (CD3E) (AA 23-207) antibody
CD3E 适用: 人, 小鼠, 大鼠, 小鸡 WB, IHC, IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 25-207) antibodyCD3E 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 23-119) antibodyCD3E 适用: 人 FACS, WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal C3e-1931 unconjugated
anti-CD3 epsilon (CD3E) (AA 156-168), (Cytoplasmic Domain) antibodyCD3E 适用: 人, 大鼠, Pig, Cow, 马, 兔, 豚鼠, 小鸡 FACS, WB, IF 宿主: 小鼠 Monoclonal PC3-188A unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E 适用: 人, 小鼠, 大鼠 FACS, IF, Func 宿主: 小鼠 Monoclonal RIV9 unconjugated
anti-CD3 epsilon (CD3E) (AA 28-185) antibodyCD3E 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 23-207) antibodyCD3E 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow_Cytometry_Dilution: 0.1-0.5 μg/10^6 cells
Other: Sufficient to run approximately 500 tests
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说明
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Anti-CD3 is tested for Flow Cytometry (Cell Surface). Researchers should determine optimal titers for applications that are not stated.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.1 % Gelatin
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
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有效期
- 6 months
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- CD3 epsilon (CD3E)
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别名
- CD3e
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背景
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Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e
Background: CD3 is a multi-subunit complex consisting of 4 different invariable membrane proteins, CD3E, CD3D, CD3G and CD3-omega. These CD3 proteins are expressed on the surface of T-cells and Thymocytes during thymocyte development. They are non-covalently associated with the TCR heterodimer and form the functional TCR-CD3 antigen receptor complex of the T-lymphocyte. CD3 expression is significant during Intrathymic T-lymphocyte maturation, TCR signaling and Thymocyte differentiation. Stimulation of TCR-CD3 complex results in activation of various protein tyrosine kinases including ZAP-70 affecting multiple cellular responses including T-cell proliferation, clonal expansion, induction of tolerance and apoptosis. Mutations in the sub-unit chains of CD3 have been associated with various immunodeficiency syndromes including SCID.
Gene Name: Cd3e
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基因ID
- 12501
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NCBI登录号
- NP_031674
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UniProt
- P22646
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途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
抗原
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