L-Selectin 抗体 (PE)
Quick Overview for L-Selectin 抗体 (PE) (ABIN6657791)
抗原
See all L-Selectin (SELL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-508-n87
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Supplier
- Rockland
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原理
- CD62L Phycoerythrin Antibody
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交叉反应 (详细)
- Cross reactivity with CD62L from other sources has not been tested.
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纯化方法
- Phycoerythrin conjugated CD62L Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD62L.
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过滤
- Sterile filtered
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免疫原
- Anti-CD62L Antibody (Monoclonal) was produced by repeated immunizations with C3H/eb mouse B lymphoma 38C-13.
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亚型
- IgG2a kappa
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Labeling Ratio
- 1-2
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应用备注
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Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)
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说明
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Anti-CD62L is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
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有效期
- 6 months
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- L-Selectin (SELL) (Selectin L (SELL))
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别名
- CD62L
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背景
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Synonyms: L-selectin CD62 antigen-like family member L Leukocyte adhesion molecule 1 LAM-1 Leukocyte-endothelial cell adhesion molecule 1 LECAM1 Lymph node homing receptor Lymphocyte antigen 22 Ly-22 Lymphocyte surface MEL-14 antigen CD62L Sell Lnhr, Ly-22, Ly22
Background: CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naive T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naive, effector, and memory T cells. CD62L has been reported to interact with CD34, glyCAM-1, and MAdCAM-1.
Gene Name: Sell
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基因ID
- 20343
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NCBI登录号
- NP_035476
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UniProt
- P18337
抗原
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