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L-Selectin 抗体 (PE)

This anti-L-Selectin antibody is a 大鼠 单克隆 antibody detecting L-Selectin in FACS, IHC 和 IP. Suitable for 小鼠.
Rockland
产品编号 ABIN6657791
Supplier Product No.: 200-508-n87
发货至: 中国

Quick Overview for L-Selectin 抗体 (PE) (ABIN6657791)

抗原

See all L-Selectin (SELL) 抗体
L-Selectin (SELL) (Selectin L (SELL))

适用

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小鼠

宿主

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大鼠

克隆类型

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单克隆

标记

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This L-Selectin antibody is conjugated to PE

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

克隆位点

MEL-14
  • Supplier Product No.

    200-508-n87

    Supplier

    Rockland

    原理

    CD62L Phycoerythrin Antibody

    交叉反应 (详细)

    Cross reactivity with CD62L from other sources has not been tested.

    纯化方法

    Phycoerythrin conjugated CD62L Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD62L.

    过滤

    Sterile filtered

    免疫原

    Anti-CD62L Antibody (Monoclonal) was produced by repeated immunizations with C3H/eb mouse B lymphoma 38C-13.

    亚型

    IgG2a kappa

    Labeling Ratio

    1-2
  • 应用备注

    Immunoprecipitation_Dilution: User Optimized

    Immunohistochemistry_Dilution: User Optimized

    Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

    说明

    Anti-CD62L is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.09 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

    有效期

    6 months
  • 抗原

    L-Selectin (SELL) (Selectin L (SELL))

    别名

    CD62L

    背景

    Synonyms: L-selectin CD62 antigen-like family member L Leukocyte adhesion molecule 1 LAM-1 Leukocyte-endothelial cell adhesion molecule 1 LECAM1 Lymph node homing receptor Lymphocyte antigen 22 Ly-22 Lymphocyte surface MEL-14 antigen CD62L Sell Lnhr, Ly-22, Ly22

    Background: CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naive T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naive, effector, and memory T cells. CD62L has been reported to interact with CD34, glyCAM-1, and MAdCAM-1.

    Gene Name: Sell

    基因ID

    20343

    NCBI登录号

    NP_035476

    UniProt

    P18337
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