L-Selectin 抗体 (APC)
Quick Overview for L-Selectin 抗体 (APC) (ABIN6657789)
抗原
See all L-Selectin (SELL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-526-n87
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Supplier
- Rockland
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原理
- CD62L Allophycocyanin Antibody
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交叉反应 (详细)
- Cross reactivity with CD62L from other sources has not been tested.
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纯化方法
- Allophycocyanin conjugated CD62L Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD62L.
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过滤
- Sterile filtered
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免疫原
- Anti-CD62L Antibody (Monoclonal) was produced by repeated immunizations with CD62L antigen.
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亚型
- IgG2a kappa
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Labeling Ratio
- 1-2
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应用备注
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Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)
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说明
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Anti-CD62L is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.1 % Gelatin
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
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有效期
- 6 months
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- L-Selectin (SELL) (Selectin L (SELL))
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别名
- CD62L
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背景
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Synonyms: L-selectin CD62 antigen-like family member L Leukocyte adhesion molecule 1 LAM-1 Leukocyte-endothelial cell adhesion molecule 1 LECAM1 Lymph node homing receptor Lymphocyte antigen 22 Ly-22 Lymphocyte surface MEL-14 antigen CD62L Sell Lnhr, Ly-22, Ly22
Background: Anti-CD62L antibody is specific for mouse CD62L, also known as L-Selectin, a cell adhesion molecule which facilitates lymphocyte rolling on activated vascular endothelium and homing to high endothelial venules (HEV) as immune cells transmigrate from blood into peripheral tissues. L-Selectin is a member of a family of Selectin molecules which act together with the integrin family of adhesion molecules to mediate leukocyte-endothelial interactions. L-Selectin is characteristically expressed by neutrophils, and is also found on B cells, monocytes, granulocytes, and at varying levels on naive, effector and memory T cells. It is rapidly shed upon cell activation, releasing into the circulation a soluble form whose biological role is of particular interest in cancer biology research.The MEL-14 antibody may be used as a phenotypic marker for CD62L expression on a variety of immune cell types. Please note that CD62L (L-Selectin) itself is also referred to as MEL-14 in the literature.
Gene Name: Sell
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基因ID
- 20343
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NCBI登录号
- NP_035476
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UniProt
- P18337
抗原
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