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L-Selectin 抗体 (APC)

This anti-L-Selectin antibody is a 大鼠 单克隆 antibody detecting L-Selectin in FACS, IHC 和 IP. Suitable for 小鼠.
Rockland
产品编号 ABIN6657789
Supplier Product No.: 200-526-n87
发货至: 中国

Quick Overview for L-Selectin 抗体 (APC) (ABIN6657789)

抗原

See all L-Selectin (SELL) 抗体
L-Selectin (SELL) (Selectin L (SELL))

适用

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小鼠

宿主

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大鼠

克隆类型

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单克隆

标记

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This L-Selectin antibody is conjugated to APC

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

克隆位点

MEL-14
  • Supplier Product No.

    200-526-n87

    Supplier

    Rockland

    原理

    CD62L Allophycocyanin Antibody

    交叉反应 (详细)

    Cross reactivity with CD62L from other sources has not been tested.

    纯化方法

    Allophycocyanin conjugated CD62L Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD62L.

    过滤

    Sterile filtered

    免疫原

    Anti-CD62L Antibody (Monoclonal) was produced by repeated immunizations with CD62L antigen.

    亚型

    IgG2a kappa

    Labeling Ratio

    1-2
  • 应用备注

    Immunoprecipitation_Dilution: User Optimized

    Immunohistochemistry_Dilution: User Optimized

    Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

    说明

    Anti-CD62L is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.1 % Gelatin

    Preservative: 0.09 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

    有效期

    6 months
  • 抗原

    L-Selectin (SELL) (Selectin L (SELL))

    别名

    CD62L

    背景

    Synonyms: L-selectin CD62 antigen-like family member L Leukocyte adhesion molecule 1 LAM-1 Leukocyte-endothelial cell adhesion molecule 1 LECAM1 Lymph node homing receptor Lymphocyte antigen 22 Ly-22 Lymphocyte surface MEL-14 antigen CD62L Sell Lnhr, Ly-22, Ly22

    Background: Anti-CD62L antibody is specific for mouse CD62L, also known as L-Selectin, a cell adhesion molecule which facilitates lymphocyte rolling on activated vascular endothelium and homing to high endothelial venules (HEV) as immune cells transmigrate from blood into peripheral tissues. L-Selectin is a member of a family of Selectin molecules which act together with the integrin family of adhesion molecules to mediate leukocyte-endothelial interactions. L-Selectin is characteristically expressed by neutrophils, and is also found on B cells, monocytes, granulocytes, and at varying levels on naive, effector and memory T cells. It is rapidly shed upon cell activation, releasing into the circulation a soluble form whose biological role is of particular interest in cancer biology research.The MEL-14 antibody may be used as a phenotypic marker for CD62L expression on a variety of immune cell types. Please note that CD62L (L-Selectin) itself is also referred to as MEL-14 in the literature.

    Gene Name: Sell

    基因ID

    20343

    NCBI登录号

    NP_035476

    UniProt

    P18337
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