CD25 抗体 (APC)
Quick Overview for CD25 抗体 (APC) (ABIN6657775)
抗原
See all CD25 (IL2RA) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-526-m73
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Supplier
- Rockland
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原理
- CD25 APC Antibody
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交叉反应 (详细)
- Reactivity is observed against mouse CD25.
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纯化方法
- Allophycocyanin conjugated CD25 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against mouse CD25.
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过滤
- Sterile filtered
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免疫原
- Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with IL-2-dependent cytolytic mouse T-cell clone B6.1.
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亚型
- IgG1 lambda
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Labeling Ratio
- 1-2
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应用备注
- Optional[Neutralization_Dilution]: 0.1-0.5 μg/1x10^6 cells
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说明
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Anti-CD25 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.1 % Gelatin
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
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有效期
- 6 months
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- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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别名
- CD25
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背景
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Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25 Antibody
Background: CD25 is a 55KDa transmembrane glycoprotein consisting of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. It is expressed during early stages of thymocytes development and in activated CD4+ memory T-lymphocytes. CD25 heterodimerizes with IL2R-beta and IL-2R gamma and forms the tripartite high affinity IL-2 receptor complex. It increases the specificity and affinity of IL-2 receptor complex for IL-2 ligand and is necessary for receptor clustering required for augmentation of IL-2 signaling. Mutations in CD25 result in severe immunodeficiency.
Gene Name: IL2RA
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基因ID
- 16184
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NCBI登录号
- NP_032393
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UniProt
- P01590
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途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
抗原
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