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CD25 抗体 (APC)

This anti-CD25 antibody is a 大鼠 单克隆 antibody detecting CD25 in FACS. Suitable for 小鼠.
Rockland
产品编号 ABIN6657775
Supplier Product No.: 200-526-m73
发货至: 中国

Quick Overview for CD25 抗体 (APC) (ABIN6657775)

抗原

See all CD25 (IL2RA) 抗体
CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

适用

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小鼠

宿主

  • 161
  • 86
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大鼠

克隆类型

  • 248
  • 81
  • 4
单克隆

标记

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This CD25 antibody is conjugated to APC

应用范围

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  • 25
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Flow Cytometry (FACS)

克隆位点

PC61
  • Supplier Product No.

    200-526-m73

    Supplier

    Rockland

    原理

    CD25 APC Antibody

    交叉反应 (详细)

    Reactivity is observed against mouse CD25.

    纯化方法

    Allophycocyanin conjugated CD25 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against mouse CD25.

    过滤

    Sterile filtered

    免疫原

    Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with IL-2-dependent cytolytic mouse T-cell clone B6.1.

    亚型

    IgG1 lambda

    Labeling Ratio

    1-2
  • 应用备注

    Optional[Neutralization_Dilution]: 0.1-0.5 μg/1x10^6 cells

    说明

    Anti-CD25 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.1 % Gelatin

    Preservative: 0.09 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

    有效期

    6 months
  • 抗原

    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

    别名

    CD25

    背景

    Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25 Antibody

    Background: CD25 is a 55KDa transmembrane glycoprotein consisting of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. It is expressed during early stages of thymocytes development and in activated CD4+ memory T-lymphocytes. CD25 heterodimerizes with IL2R-beta and IL-2R gamma and forms the tripartite high affinity IL-2 receptor complex. It increases the specificity and affinity of IL-2 receptor complex for IL-2 ligand and is necessary for receptor clustering required for augmentation of IL-2 signaling. Mutations in CD25 result in severe immunodeficiency.

    Gene Name: IL2RA

    基因ID

    16184

    NCBI登录号

    NP_032393

    UniProt

    P01590

    途径

    JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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