Cytokeratin 18 抗体
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北京 101111
Quick Overview for Cytokeratin 18 抗体 (ABIN6657540)
抗原
See all Cytokeratin 18 (KRT18) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-p33
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Supplier
- Rockland
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原理
- Cytokeratin Peptide 18 Antibody
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交叉反应 （详细）
- This product reacts with human and rat.
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纯化方法
- Anti-Cytokeratin Peptide 18 antibody was purified from mouse ascites by protein A chromatography.
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免疫原
- Cytokeratin Peptide 18 antibody was produced in mice by repeated immunizations with the human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.
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亚型
- IgG1
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anti-Keratin 18 (KRT18) (AA 53-150) antibody
KRT18 适用: 人, 小鼠, 大鼠, Pig WB, FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) antibodyKRT18 适用: 哺乳动物 WB, ELISA, IHC (p), ICC, IP, ICFC 宿主: 小鼠 Monoclonal C-04 unconjugated
anti-Keratin 18 (KRT18) antibodyKRT18 适用: 人 WB, ELISA, IHC (p), ICC, IP, ICFC 宿主: 小鼠 Monoclonal DC-10 unconjugated
anti-Keratin 18 (KRT18) (AA 1-423) antibodyKRT18 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 238-396) antibodyKRT18 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 238-396) antibodyKRT18 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal E4 unconjugated
anti-Keratin 18 (KRT18) (AA 72-380) antibodyKRT18 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 1-430) antibodyKRT18 适用: 人 WB, IF, ELISA, IHC (p), IP 宿主: 小鼠 Monoclonal 2F8 unconjugated
anti-Keratin 18 (KRT18) (AA 102-396) antibodyKRT18 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 1-150) antibodyKRT18 适用: 人 WB, IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 6G12F2 unconjugated
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应用备注
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Immunohistochemistry_Dilution: 0.5-2 μg/mL
Western_Blot_Dilution: 0.5-1 μg/mL
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说明
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Anti-Cytokeratin Peptide 18 is tested for Immunohistochemistry-F, Immunohistochemistry-P, Immunofluorescence, and Western Blot. Expect a band approximately ~48 kDa corresponding to the appropriate cell lysate or extract. western blot tested in human. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Neutral PBS
Reconstitution_Volume: 1.0 mL
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缓冲液
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Buffer: 1.2 % sodium acetate
Stabilizer: 2 mg BSA
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
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别名
- Cytokeratin Peptide 18
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背景
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Synonyms: K18, Keratin-18, CK-18, Cytokeratin-18, CYK18, Cell proliferation-inducing gene 46 protein
Background: Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis. This antibody is suitable for researchers interested in cancer research, cardiovascular diseases, and cytoskeletal signaling research.
Gene Name: KRT18
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基因ID
- 3875
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NCBI登录号
- NP_000215
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UniProt
- P05783
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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