Cyclin A 抗体
Quick Overview for Cyclin A 抗体 (ABIN6657484)
抗原
See all Cyclin A (CCNA2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-p30
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Supplier
- Rockland
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原理
- Cyclin A Antibody
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交叉反应 (详细)
- This product reacts with human, mouse, and bovine.
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纯化方法
- Anti-Cyclin A antibody was purified from mouse ascites by protein A chromatography.
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免疫原
- Cyclin A antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to bovine cyclin A.
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亚型
- IgG2a
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应用备注
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ELISA_Dilution: 1:10,000
Immunohistochemistry_Dilution: 1:100-1:500
Western_Blot_Dilution: 0.5-1 μg/mL
Other: Immunocytochemistry : 1 μg/mL
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说明
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Anti-Cyclin A is tested for Immunocytochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~48 kDa corresponding to the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Neutral PBS
Reconstitution_Volume: 1.0 mL
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缓冲液
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Buffer: 1.2 % sodium acetate
Stabilizer: 2 mg BSA
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Cyclin A (CCNA2) (Cyclin A2 (CCNA2))
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别名
- Cyclin A
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背景
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Synonyms: CCN1, CCNA
Background: The mammalian A-type cyclin family consists of 2 members, cyclin A1 (CCNA1) and cyclin A2 (CCNA2). Human cyclin A gene is assigned to 4q26-q27. Cyclin A protein is synthesized and localized into the nucleus at the onset of S phase in nontransformed mammalian fibroblasts. This antibody is suitable for researchers interested in cancer research, apoptosis, autoimmune disease, neurodegenerative disease, cardiovascular disease, MAP Kinase Signaling, AKT Signaling, Inflammatory Signaling, and Cell Cycle research.
Gene Name: CCNA2
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基因ID
- 281667
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NCBI登录号
- NP_001068591
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UniProt
- P30274
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途径
- PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
抗原
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