ALOX5 抗体 (pSer523)
Quick Overview for ALOX5 抗体 (pSer523) (ABIN6657368)
抗原
See all ALOX5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer523
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Supplier Product No.
- 600-401-d03
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Supplier
- Rockland
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原理
- 5-Lipoxygenase phospho S523 Antibody
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交叉反应 （详细）
- Anti-5-Lipoxygenase pS523 Antibody is directed against human 5-Lipoxygenase phosphorylated at S523.
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纯化方法
- The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
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免疫原
- Anti-5-Lipoxygenase pS523 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser523.
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亚型
- IgG
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anti-Arachidonate 5-Lipoxygenase (ALOX5) (AA 461-671) antibody
ALOX5 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Arachidonate 5-Lipoxygenase (ALOX5) (AA 1-674) antibodyALOX5 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Arachidonate 5-Lipoxygenase (ALOX5) (AA 1-300) antibodyALOX5 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Arachidonate 5-Lipoxygenase (ALOX5) antibodyALOX5 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Arachidonate 5-Lipoxygenase (ALOX5) (AA 120-483) antibodyALOX5 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Arachidonate 5-Lipoxygenase (ALOX5) (AA 601-674) antibodyALOX5 适用: 人, 小鼠, 大鼠 IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Arachidonate 5-Lipoxygenase (ALOX5) (C-Term) antibodyALOX5 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Arachidonate 5-Lipoxygenase (ALOX5) (AA 461-671) antibodyALOX5 适用: 人 WB 宿主: 小鼠 Monoclonal D10 unconjugated
anti-Arachidonate 5-Lipoxygenase (ALOX5) (AA 2-149) antibodyALOX5 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Arachidonate 5-Lipoxygenase (ALOX5) antibodyALOX5 适用: 人 IHC, ELISA 宿主: 兔 Monoclonal 1D4 unconjugated Recombinant Antibody
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应用备注
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ELISA_Dilution: 1:10,000
Western_Blot_Dilution: 1:1000
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说明
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Anti-5-Lipoxygenase pS523 antibody has been tested in Western Blotting and is suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 80 kDa corresponding to the 80 kDa 5-LO proteins phosphorylated at Ser523.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))
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别名
- 5-Lipoxygenase
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背景
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Synonyms: Arachidonate 5-lipoxygenase, 5-LO
Background: 5-Lipoxygenase pS523 antibody recognizes the enzyme 5-lipoxygenase (5-LO) which plays a key role in regulating the production of leukotrienes (LTs). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma, fibrosis and atherosclerosis. Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO. Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis. Anti-5-Lipoxygenase pS523 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Gene Name: ALOX5
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基因ID
- 240
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UniProt
- P09917
抗原
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