HSPD1 抗体
Quick Overview for HSPD1 抗体 (ABIN6657342)
抗原
See all HSPD1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-f60
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Supplier
- Rockland
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原理
- HSP60 Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with Hsp60 from Human, Mouse, Rat, Bovine, Canine, Chicken, Drosophila, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, Plant, Silkworm, and Xenopus sources based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-HSP60 Antibody was purified by Protein G chromatography.
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过滤
- Sterile filtered
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免疫原
- HSP60 Antibody was produced in mice by repeated immunizations raised against recombinant human Hsp60.
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亚型
- IgG1
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anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 501-573) antibody
HSPD1 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 27-240) antibodyHSPD1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibodyHSPD1 适用: 人 WB, IHC, FACS, ELISA, IP 宿主: 小鼠 Monoclonal LK1 unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibodyHSPD1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 3G8 unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 1-573) antibodyHSPD1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 172-343) antibodyHSPD1 适用: 人 WB, IHC, FACS, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 316-573) antibodyHSPD1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibodyHSPD1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IEM 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 1-573) antibodyHSPD1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibodyHSPD1 适用: 人, 小鼠, 大鼠, 小鸡, 猴, Hamster, 犬, Cow, 兔, Pig, 绵羊, Drosophila melanogaster, 非洲爪蟾 WB, IHC, IF, FACS, ISt, StM 宿主: 小鼠 Monoclonal LK1 unconjugated
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应用备注
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Immunoprecipitation_Dilution: 1:200
ELISA_Dilution: 1:200
Immunohistochemistry_Dilution: 1:100
Flow_Cytometry_Dilution: User Optimized
IF_Microscopy_Dilution: 1:100
Western_Blot_Dilution: 1:20000
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说明
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Anti-Hsp60 Antibody is tested for WB, IP, ELISA, Flow Cytometry, and IHC. Expect a band approximately ~60kDa protein corresponding to the molecular mass of Hsp60 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
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别名
- Hsp60
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背景
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Synonyms: 60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, Mitochondrial matrix protein P1, P60 lymphocyte protein, PD1, CPN60 Antibody, GROEL Antibody, HLD4 Antibody, HSP 60 Antibody, HSP65 Antibody, HSPD1 Antibody, HuCHA60 Antibody, SPG 13 Antibody
Background: In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. Hsp60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian Hsp60. Whereas mammalian Hsp60 is localized within the mitochondria, plant Hsp60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that Hsp60 is present in so many different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures. These similarities are supported by recent studies where the single-ring human mitochondrial homolog, Hsp60 with its co-chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES. Another important function of Hsp60 and Hsp10 is their protective functions against infection and cellular stress. Hsp60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes. Anti-Hsp60 Antibody is useful for researchers interested in Heat Shock research.
Gene Name: HSPD1
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基因ID
- 3329
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NCBI登录号
- NP_002147
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UniProt
- P10809
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途径
- Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
抗原
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