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WASF1 抗体 (C-Term)

This 小鼠 单克隆 antibody specifically detects WASF1 in WB, IHC, IP 和 FM. It exhibits reactivity toward 人.
Rockland
产品编号 ABIN6656415
Supplier Product No.: 200-301-g64
发货至: 中国
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Quick Overview for WASF1 抗体 (C-Term) (ABIN6656415)

抗原

See all WASF1 抗体
WASF1 (WAS Protein Family, Member 1 (WASF1))

适用

  • 51
  • 20
  • 10
  • 1

宿主

  • 44
  • 7
  • 1
  • 1
小鼠

克隆类型

  • 44
  • 9
单克隆

标记

  • 24
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This WASF1 antibody is un-conjugated

应用范围

  • 37
  • 23
  • 21
  • 13
  • 13
  • 10
  • 7
  • 6
  • 3
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

克隆位点

S91-36
  • 抗原表位

    • 15
    • 14
    • 6
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    C-Term

    Supplier Product No.

    200-301-g64

    Supplier

    Rockland

    原理

    WAVE1-Scar Antibody

    交叉反应 (详细)

    A BLAST analysis was used to suggest cross-reactivity with WAVE1-Scar from Mouse, Human, and Rat based on 100 % homology with the immunizing sequence.

    纯化方法

    Anti-WAVE1-Scar Antibody was purified by Protein G chromatography.

    过滤

    Sterile filtered

    免疫原

    WAVE1-Scar Antibody was produced in mice by repeated immunizations raised against a synthetic peptide corresponding to the near c-terminal region of human Wave1/Wasp family1.

    亚型

    IgG1
  • 应用备注

    Immunoprecipitation_Dilution: User Optimized

    Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

    IF_Microscopy_Dilution: 1.0-10 μg/mL

    Western_Blot_Dilution: 1 μg/mL

    说明

    Anti-WAVE1-Scar Antibody is suitable for use in WB, IHC and IP. Expect a band approximately 75kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    Preservative: 0.09 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • 抗原

    WASF1 (WAS Protein Family, Member 1 (WASF1))

    别名

    WAVE1-Scar

    背景

    Synonyms: Wave1, Scar1, WASP, Wave, WASF1, Wiskott-Aldrich syndrome protein family member 1, WASP family protein member 1, Verprolin homology domain-containing protein 1

    Background: The Wiskott-Aldrich syndrome protein (WASP) family plays essential roles in the regulation of actin polymerization. There are five WASP family members: Wasp, N-WASP, Wave1 (WASP family verprolin-homologous protein 1), WAVE2 and WAVE3. The Wasp family proteins share a conserved C-terminal region called the verprolin homology, cofilin homology and acidic domain. The Wave/Scar family of prtoteins mediates signals to actin assembly by direct activation of the Arp2/3 complex. These proteins have been characterized as major regulators of lamellipodia formation downstream of Rac activation, and as members of large protein complexes. IT has been found that all three Scar/Wave isoforms behave similarily and are likely to participate in the same kinds of protein complexes.

    Gene Name: WASF1

    基因ID

    8936

    NCBI登录号

    NP_001020105

    UniProt

    Q92558

    途径

    RTK signaling, Regulation of Actin Filament Polymerization
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