WASF1 抗体 (C-Term)
Quick Overview for WASF1 抗体 (C-Term) (ABIN6656415)
抗原
See all WASF1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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Supplier Product No.
- 200-301-g64
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Supplier
- Rockland
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原理
- WAVE1-Scar Antibody
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交叉反应 (详细)
- A BLAST analysis was used to suggest cross-reactivity with WAVE1-Scar from Mouse, Human, and Rat based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-WAVE1-Scar Antibody was purified by Protein G chromatography.
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过滤
- Sterile filtered
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免疫原
- WAVE1-Scar Antibody was produced in mice by repeated immunizations raised against a synthetic peptide corresponding to the near c-terminal region of human Wave1/Wasp family1.
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亚型
- IgG1
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应用备注
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Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: 0.1-1.0 μg/mL
IF_Microscopy_Dilution: 1.0-10 μg/mL
Western_Blot_Dilution: 1 μg/mL
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说明
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Anti-WAVE1-Scar Antibody is suitable for use in WB, IHC and IP. Expect a band approximately 75kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- WASF1 (WAS Protein Family, Member 1 (WASF1))
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别名
- WAVE1-Scar
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背景
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Synonyms: Wave1, Scar1, WASP, Wave, WASF1, Wiskott-Aldrich syndrome protein family member 1, WASP family protein member 1, Verprolin homology domain-containing protein 1
Background: The Wiskott-Aldrich syndrome protein (WASP) family plays essential roles in the regulation of actin polymerization. There are five WASP family members: Wasp, N-WASP, Wave1 (WASP family verprolin-homologous protein 1), WAVE2 and WAVE3. The Wasp family proteins share a conserved C-terminal region called the verprolin homology, cofilin homology and acidic domain. The Wave/Scar family of prtoteins mediates signals to actin assembly by direct activation of the Arp2/3 complex. These proteins have been characterized as major regulators of lamellipodia formation downstream of Rac activation, and as members of large protein complexes. IT has been found that all three Scar/Wave isoforms behave similarily and are likely to participate in the same kinds of protein complexes.
Gene Name: WASF1
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基因ID
- 8936
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NCBI登录号
- NP_001020105
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UniProt
- Q92558
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途径
- RTK signaling, Regulation of Actin Filament Polymerization
抗原
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