PINK1 抗体 (AA 112-496)
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- 抗原 See all PINK1 抗体
- PINK1 (PTEN Induced Putative Kinase 1 (PINK1))
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抗原表位
- AA 112-496
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This PINK1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)
- Supplier Product No.
- 200-301-w64
- Supplier
- Rockland
- 原理
- PINK1 Antibody
- 交叉反应 （详细）
- BLAST analysis suggests that it is 82 % identical to rat, 81 % identical to mouse and >30 % identical to DMPK.
- 纯化方法
- Anti-PINK1 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.
- 过滤
- Sterile filtered
- 免疫原
- Anti-PINK1 Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 112-496 of human PINK1.
- 克隆位点
- S4-15
- 亚型
- IgG1
- Top Product
- Discover our top product PINK1 Primary Antibody
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anti-PTEN Induced Putative Kinase 1 (PINK1) (Internal Region) antibody
Verified PINK1 适用: 人 WB, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-273) antibodyPINK1 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 3A9F8 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) antibodyPINK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 小鼠 Monoclonal 38CT20-8-5 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-273) antibodyPINK1 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5F2B11 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibodyPINK1 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S4-15 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (HRP)PINK1 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S4-15 HRP
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (FITC)PINK1 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S4-15 FITC
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (Atto 594)PINK1 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S4-15 Atto 594
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (Atto 390)PINK1 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S4-15 Atto 390
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (Biotin)PINK1 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S4-15 Biotin
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- 应用备注
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ELISA_Dilution: 1:10,000
IF_Microscopy_Dilution: User Optimized
Western_Blot_Dilution: 1:1000
Other: Immunocytochemistry/Immunofluorescence 1:100
- 说明
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Anti-PINK1 Antibody has been tested in WB, IHC, and ICC/IF. Expect a band approximately ~50 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.1 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- PINK1 (PTEN Induced Putative Kinase 1 (PINK1))
- 别名
- PINK1 (PINK1 产品)
- 背景
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Synonyms: Parkinson disease (autosomal recessive) 6, EC 2.7.11.1, PARK6, serine/threonine-protein kinase PINK1 mitochondrial, PTEN-induced putative kinase protein 1, protein kinase BRPK, BRPK, FLJ27236, PTEN induced putative kinase 1, Phosphatase and Tensin Homolog
Background: PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 is synthesized as a 63 kD protein which undergoes proteolyt processing to generate at least two cleaved forms (55 kD and 42 kD). PINK1 and its substrates have been found in the cytosol as well as in different sub-mitochondrial compartments, and according to the recent reports, PINK1 may be targeted to OMM (outer mitochondrial membrane) with its kinase domain facing the cytosol, providing a possible explanation for the observed physical interaction with the cytosolic E3 ubiquitin ligase Parkin. Defective PINK1 may cause alterations in processing, stability, localization and activity as well as binding to substrates/interaction-partners which ultimately leads to differential effects on mitochondrial function and morphology. Mutations in PINK1 are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of alpha-synuclein, as well as proteasome dysfunction. Areas of interest and use for researchers include Neuroscience, mitochondrial function, and CDK-mediated phosphorylation pathways.
Gene Name: PINK1
- 基因ID
- 65018
- NCBI登录号
- NP_115785
- 途径
- Autophagy
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