Olfactomedin 1 抗体 (Internal Region)
Quick Overview for Olfactomedin 1 抗体 (Internal Region) (ABIN6656290)
抗原
See all Olfactomedin 1 (OLFM1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Internal Region
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Supplier Product No.
- 200-301-g28
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Supplier
- Rockland
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原理
- Pancortin Antibody
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交叉反应 (详细)
- A BLAST analysis was used to suggest cross-reactivity with Pancortin from Mouse, Human, and Rat based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-Pancortin Antibody was purified by Protein G chromatography.
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过滤
- Sterile filtered
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免疫原
- Pancortin Antibody was produced in mice by repeated immunizations raised against a synthetic peptide corresponding to an internal region of human pancortin-1.
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亚型
- IgG1
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应用备注
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Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: 0.1-1.0 μg/mL
IF_Microscopy_Dilution: 1.0-10 μg/mL
Western_Blot_Dilution: 1 μg/mL
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说明
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Anti-Pancortin Antibody is tested for use in WB, IHC and IP. Expect a band approximately ~70kDa (Pancortin-1, Pancortin-3) and ~20kDa (Pancortin-2 and Pancortin-4) on specific lysates. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Olfactomedin 1 (OLFM1)
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别名
- Pancortin
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背景
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Synonyms: Noelin, AMY, Noe1, Olfm1, Olfactomedin1, Neuronal olfactomedin-related ER localized protein, Pancortin
Background: Pancortin, a member of a diverse family of secreted glycoproteins, is implicated in the development of the nervous system, and the glaucoma-associated protein myocilin. Studies suggest that Pancortin-2 interact with WAVE1 in a novel cell death cascade in adult, but not embryonic, cerebral cortical neurons. Specifically pancortin-2 is a mediator of ischemia-induced apoptosis of neurons in the adult cerebral cortex, and functions in a novel mitrochondrial/actin associated protein complex that sequesters Bcl-xL. It is also involved in the generation of neural crest cells, and in neurogenesis.
Gene Name: Olfm1
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基因ID
- 10439
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NCBI登录号
- NP_001269540
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UniProt
- Q99784
抗原
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