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Olfactomedin 1 抗体 (Internal Region)

The 小鼠 单克隆 anti-Olfactomedin 1 antibody is suitable to detect Olfactomedin 1 in samples from 人. It has been validated for WB, IHC, FM 和 IP.
Rockland
产品编号 ABIN6656290
Supplier Product No.: 200-301-g28
发货至: 中国
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Quick Overview for Olfactomedin 1 抗体 (Internal Region) (ABIN6656290)

抗原

See all Olfactomedin 1 (OLFM1) 抗体
Olfactomedin 1 (OLFM1)

适用

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宿主

  • 63
  • 9
  • 1
小鼠

克隆类型

  • 64
  • 9
单克隆

标记

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This Olfactomedin 1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Immunoprecipitation (IP)

克隆位点

S96-7
  • 抗原表位

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    Internal Region

    Supplier Product No.

    200-301-g28

    Supplier

    Rockland

    原理

    Pancortin Antibody

    交叉反应 (详细)

    A BLAST analysis was used to suggest cross-reactivity with Pancortin from Mouse, Human, and Rat based on 100 % homology with the immunizing sequence.

    纯化方法

    Anti-Pancortin Antibody was purified by Protein G chromatography.

    过滤

    Sterile filtered

    免疫原

    Pancortin Antibody was produced in mice by repeated immunizations raised against a synthetic peptide corresponding to an internal region of human pancortin-1.

    亚型

    IgG1
  • 应用备注

    Immunoprecipitation_Dilution: User Optimized

    Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

    IF_Microscopy_Dilution: 1.0-10 μg/mL

    Western_Blot_Dilution: 1 μg/mL

    说明

    Anti-Pancortin Antibody is tested for use in WB, IHC and IP. Expect a band approximately ~70kDa (Pancortin-1, Pancortin-3) and ~20kDa (Pancortin-2 and Pancortin-4) on specific lysates. Specific conditions for reactivity should be optimized by the end user.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    Preservative: 0.09 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • 抗原

    Olfactomedin 1 (OLFM1)

    别名

    Pancortin

    背景

    Synonyms: Noelin, AMY, Noe1, Olfm1, Olfactomedin1, Neuronal olfactomedin-related ER localized protein, Pancortin

    Background: Pancortin, a member of a diverse family of secreted glycoproteins, is implicated in the development of the nervous system, and the glaucoma-associated protein myocilin. Studies suggest that Pancortin-2 interact with WAVE1 in a novel cell death cascade in adult, but not embryonic, cerebral cortical neurons. Specifically pancortin-2 is a mediator of ischemia-induced apoptosis of neurons in the adult cerebral cortex, and functions in a novel mitrochondrial/actin associated protein complex that sequesters Bcl-xL. It is also involved in the generation of neural crest cells, and in neurogenesis.

    Gene Name: Olfm1

    基因ID

    10439

    NCBI登录号

    NP_001269540

    UniProt

    Q99784
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