EZH2 抗体 (N-Term)
Quick Overview for EZH2 抗体 (N-Term) (ABIN6656040)
抗原
See all EZH2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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Supplier Product No.
- 200-401-w53
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Supplier
- Rockland
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原理
- EzH2 Antibody
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交叉反应 (详细)
- Cross reactivity with other species was not tested.
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纯化方法
- Anti-EZH2 Antibody was purified by Protein G chromatography.
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过滤
- Sterile filtered
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免疫原
- Anti-EZH2 Antibody was produced in rabbits by repeated immunizations containing an amino acid sequence from the N-terminus of mouse EZH2.
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亚型
- IgG
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应用备注
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ChIP_Dilution: 2.5 μg per IP
Immunohistochemistry_Dilution: 1:100 - 1:500
Western_Blot_Dilution: 1:1,000
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说明
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Anti-EZH2 Antibody is tested for Chromatin Immunoprecipitation, Immunofluorescence, and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 85 kDa in the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300 -
储存液
- ProClin, Sodium azide
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注意事项
- This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- EZH2 (Enhancer of Zeste Homolog 2 (EZH2))
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别名
- EzH2
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背景
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Synonyms: Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2
Background: EZH2 is a histone-lysine methyltransferase which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression. It is a member of the polycomb group (PcG) family which form multimeric protein complexes and are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2 activity is dependent on the association with other components of the PRC2 complex (EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7). EZH2 may play a role in the hematopoietic and internal nervous systems. Over-expression of EZH2 is observed during advanced stages of prostate cancer and breast cancer. Anti-EZH2 Antibody is ideal for research in Epigenetics, Gene Expression, Cell Biology and Cancer.
Gene Name: EZH2
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基因ID
- 2146
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NCBI登录号
- NP_001190176
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UniProt
- Q15910
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途径
- Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation
抗原
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