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EZH2 抗体 (N-Term)

This anti-EZH2 antibody is a 兔 多克隆 antibody detecting EZH2 in WB, IHC, IF 和 ChIP. Suitable for 人.
Rockland
产品编号 ABIN6656040
Supplier Product No.: 200-401-w53
发货至: 中国

Quick Overview for EZH2 抗体 (N-Term) (ABIN6656040)

抗原

See all EZH2 抗体
EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

适用

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宿主

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克隆类型

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多克隆

标记

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This EZH2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)
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    N-Term

    Supplier Product No.

    200-401-w53

    Supplier

    Rockland

    原理

    EzH2 Antibody

    交叉反应 (详细)

    Cross reactivity with other species was not tested.

    纯化方法

    Anti-EZH2 Antibody was purified by Protein G chromatography.

    过滤

    Sterile filtered

    免疫原

    Anti-EZH2 Antibody was produced in rabbits by repeated immunizations containing an amino acid sequence from the N-terminus of mouse EZH2.

    亚型

    IgG
  • 应用备注

    ChIP_Dilution: 2.5 μg per IP

    Immunohistochemistry_Dilution: 1:100 - 1:500

    Western_Blot_Dilution: 1:1,000

    说明

    Anti-EZH2 Antibody is tested for Chromatin Immunoprecipitation, Immunofluorescence, and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 85 kDa in the appropriate cell lysate or extract.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

    储存液

    ProClin, Sodium azide

    注意事项

    This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • 抗原

    EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

    别名

    EzH2

    背景

    Synonyms: Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2

    Background: EZH2 is a histone-lysine methyltransferase which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression. It is a member of the polycomb group (PcG) family which form multimeric protein complexes and are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2 activity is dependent on the association with other components of the PRC2 complex (EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7). EZH2 may play a role in the hematopoietic and internal nervous systems. Over-expression of EZH2 is observed during advanced stages of prostate cancer and breast cancer. Anti-EZH2 Antibody is ideal for research in Epigenetics, Gene Expression, Cell Biology and Cancer.

    Gene Name: EZH2

    基因ID

    2146

    NCBI登录号

    NP_001190176

    UniProt

    Q15910

    途径

    Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation
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