MAPK14 抗体
Quick Overview for MAPK14 抗体 (ABIN6655671)
抗原
See all MAPK14 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-101-hb4s
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Supplier
- Rockland
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原理
- P38 Antibody
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交叉反应 (详细)
- This affinity purified antibody is directed against human p38.
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纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-p38 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human p38 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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应用备注
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ELISA_Dilution: 5 μg/mL
Immunohistochemistry_Dilution: User Optimized
IF_Microscopy_Dilution: 15 μg/mL
Western_Blot_Dilution: 1:1000
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说明
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Anti-p38 Antibody has been tested in Western Blot, ELISA and Immunohistochemistry. Expect a band at ~42 kDa in western blot using appropriate lysates. Positive control whole cell lysates used NIH3T3 and PC-12 @ 1μg/mL for WB, breast cancer tissue for IHC. This antibody has been additionally validated using a p38 knock-out lysate.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
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别名
- P38
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背景
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Synonyms: Goat Anti-p38 Antibody, Goat Anti-MAPK14 Antibody, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, MAPK14
Background: p38, also called SAPK2A, belongs to the MAP kinase family. The MAP kinases are critical signaling proteins for many biochemical signals. They participate in transcription regulation, cellular differentiation and proliferation. Environmental signals and cytokines catalyze the kinases into bonding their substrates such as MAX and ATF2, for example, and p53 a tumor suppressing gene. p38 may be associated with diseases such as chlamydia and patellar tendinitis. Anti-p38 antibody is useful for researchers interested in signal pathways, cellular development or cancer research.
Gene Name: MAPK14
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基因ID
- 1432
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NCBI登录号
- NP_001306
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UniProt
- Q16539
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途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins
抗原
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