CD27 抗体 (FITC)
Quick Overview for CD27 抗体 (FITC) (ABIN6655550)
抗原
See all CD27 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-302-n65
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Supplier
- Rockland
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原理
- CD27 Fluorescein Antibody
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交叉反应 (详细)
- Reactivity is observed against human CD27, Baboon, Cynomolgus, Rhesus, and Squirrel Monkey.
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纯化方法
- Fluorescein conjugated CD27 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD27.
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过滤
- Sterile filtered
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免疫原
- Anti-CD27 Antibody (Monoclonal) was produced by repeated immunizations with CD27 antigen.
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亚型
- IgG1 kappa
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Labeling Ratio
- 4-6
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应用备注
- Optional[Neutralization_Dilution]: 5 µL/1x10e6 cells or 100μL of whole blood
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说明
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Anti-CD27 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.2 % BSA (w/v)
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
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有效期
- 6 months
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: "Direct cellular communications between CD45R0 and CD45RA T cell subsets via CD27/CD70." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3627-35, (1995) (PubMed).
: "CD27: marker and mediator of T-cell activation?" in: Immunology today, Vol. 15, Issue 7, pp. 307-11, (1994) (PubMed).
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: "Direct cellular communications between CD45R0 and CD45RA T cell subsets via CD27/CD70." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3627-35, (1995) (PubMed).
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- CD27 (CD27 Molecule (CD27))
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别名
- CD27
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背景
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Synonyms: CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7
Background: CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-kB and SAPK/JNK activation.
Gene Name: CD27
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基因ID
- 939
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NCBI登录号
- NP_001233
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UniProt
- P26842
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途径
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
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