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CD27 抗体 (FITC)

This anti-CD27 antibody is a 小鼠 单克隆 antibody detecting CD27 in FACS. Suitable for 人. This Primary Antibody has been cited in 2+ publications.
Rockland
产品编号 ABIN6655550
Supplier Product No.: 200-302-n65
发货至: 中国

Quick Overview for CD27 抗体 (FITC) (ABIN6655550)

抗原

See all CD27 抗体
CD27 (CD27 Molecule (CD27))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD27 antibody is conjugated to FITC

应用范围

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Flow Cytometry (FACS)

克隆位点

O323
  • Supplier Product No.

    200-302-n65

    Supplier

    Rockland

    原理

    CD27 Fluorescein Antibody

    交叉反应 (详细)

    Reactivity is observed against human CD27, Baboon, Cynomolgus, Rhesus, and Squirrel Monkey.

    纯化方法

    Fluorescein conjugated CD27 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD27.

    过滤

    Sterile filtered

    免疫原

    Anti-CD27 Antibody (Monoclonal) was produced by repeated immunizations with CD27 antigen.

    亚型

    IgG1 kappa

    Labeling Ratio

    4-6
  • 应用备注

    Optional[Neutralization_Dilution]: 5 µL/1x10e6 cells or 100μL of whole blood

    说明

    Anti-CD27 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.2 % BSA (w/v)

    Preservative: 0.09 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

    有效期

    6 months
  • Agematsu, Kobata, Sugita, Hirose, Schlossman, Morimoto: "Direct cellular communications between CD45R0 and CD45RA T cell subsets via CD27/CD70." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3627-35, (1995) (PubMed).

    Hintzen, de Jong, Lens, van Lier: "CD27: marker and mediator of T-cell activation?" in: Immunology today, Vol. 15, Issue 7, pp. 307-11, (1994) (PubMed).

  • 抗原

    CD27 (CD27 Molecule (CD27))

    别名

    CD27

    背景

    Synonyms: CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7

    Background: CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-kB and SAPK/JNK activation.

    Gene Name: CD27

    基因ID

    939

    NCBI登录号

    NP_001233

    UniProt

    P26842

    途径

    Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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