CD27 抗体 (APC)
Quick Overview for CD27 抗体 (APC) (ABIN6655549)
抗原
See all CD27 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-326-n65
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Supplier
- Rockland
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原理
- CD27 Allophycocyanin Antibody
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交叉反应 （详细）
- Reactivity is observed against human CD27, Baboon, Cynomolgus, and Rhesus.
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纯化方法
- Allophycocyanin conjugated CD27 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD27.
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过滤
- Sterile filtered
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免疫原
- Anti-CD27 Antibody (Monoclonal) was produced by repeated immunizations with CD27 antigen.
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亚型
- IgG1 kappa
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Labeling Ratio
- 1-2
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anti-CD27 Molecule (CD27) (AA 1-260) antibody
CD27 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 1E2-A3 unconjugated
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人 FACS, ELISA, IHC, IF, StM, Coat 宿主: 小鼠 Monoclonal LPFS2-1611 unconjugated
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) (AA 20-191) antibodyCD27 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) (AA 20-144) antibodyCD27 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 3B6D3 unconjugated
anti-CD27 Molecule (CD27) (AA 196-225) antibodyCD27 适用: 人 FACS, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) (AA 229-312) antibodyCD27 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人 FACS, IHC, StM 宿主: 小鼠 Monoclonal rLPFS2-1611 unconjugated Recombinant Antibody
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人 FACS 宿主: 小鼠 Monoclonal LT27 unconjugated
anti-CD27 Molecule (CD27) (AA 20-191) antibodyCD27 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM57 unconjugated Recombinant Antibody
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应用备注
- Optional[Neutralization_Dilution]: 5μL/test/1x10e5 to 1x10e8 cells
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说明
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Anti-CD27 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.1 % Gelatin
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
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有效期
- 6 months
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- CD27 (CD27 Molecule (CD27))
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别名
- CD27
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背景
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Synonyms: CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7
Background: CD27 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells. The CD27 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish naive vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD27, CD1d, IL-10 and TGF-beta.
Gene Name: CD27
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基因ID
- 939
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NCBI登录号
- NP_001233
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UniProt
- P26842
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途径
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
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