Beclin 1 抗体 (pSer234)
(1 reference)
Quick Overview for Beclin 1 抗体 (pSer234) (ABIN6655419)
抗原
See all Beclin 1 (BECN1) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- pSer234
-
Supplier Product No.
- 600-401-w96
-
Supplier
- Rockland
-
原理
- BECLIN1 phospho S234 Antibody
-
交叉反应 （详细）
- Anti-Beclin1 pS234 Antibody is directed against Beclin 1 protein phosphorylated at S234.
-
纯化方法
- The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
-
过滤
- Sterile filtered
-
免疫原
- Anti-Phospho-Ser234 Beclin-1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 of human Beclin-1 conjugated to KLH.
-
亚型
- IgG
-
-
anti-Beclin 1, Autophagy Related (BECN1) (AA 201-330) antibody
BECN1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Beclin 1, Autophagy Related (BECN1) antibodyBECN1 适用: 人, 大鼠, 犬 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1F1 unconjugated
anti-Beclin 1, Autophagy Related (BECN1) antibodyBECN1 适用: 人, 大鼠, 猴 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 3F3 unconjugated
anti-Beclin 1, Autophagy Related (BECN1) antibodyBECN1 适用: 人, 大鼠, 猴, 犬 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 3C3 unconjugated
anti-Beclin 1, Autophagy Related (BECN1) antibodyBECN1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 2A4 unconjugated
anti-Beclin 1, Autophagy Related (BECN1) (AA 1-354) antibodyBECN1 适用: 人, 小鼠, 大鼠 WB, IF, FACS, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Beclin 1, Autophagy Related (BECN1) (AA 210-239) antibodyBECN1 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB7463 unconjugated
anti-Beclin 1, Autophagy Related (BECN1) (AA 210-239) antibodyBECN1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Beclin 1, Autophagy Related (BECN1) (AA 1-273) antibodyBECN1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Beclin 1, Autophagy Related (BECN1) (C-Term) antibodyBECN1 适用: 人, 小鼠 WB, ELISA, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
ELISA_Dilution: 1:2,500
Western_Blot_Dilution: 1:250
-
说明
-
Suggested Applications: ELISA
Anti-Phospho-Ser234 Beclin-1 Antibody is tested for WB. Expect a band at approximately ~60 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user. Immunolabeling of the Beclin-1 band is decreased in the Beclin-1 alanine substitution mutant. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
-
Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
Preservative: None -
储存液
- Without preservative
-
储存条件
- -20 °C
-
储存方法
- Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
-
: "Assessment of Posttranslational Modifications of ATG proteins." in: Methods in enzymology, Vol. 587, pp. 171-188, (2017) (PubMed).
-
: "Assessment of Posttranslational Modifications of ATG proteins." in: Methods in enzymology, Vol. 587, pp. 171-188, (2017) (PubMed).
-
- Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
-
别名
- Beclin1
-
背景
-
Synonyms: Run domain Beclin-1 interacting and cysteine-rich containing protein, Rubicon, Beclin-1 associated RUN domain containing protein
Background: Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies. Beclin-1 has been shown to interact with 14-3-3 proteins through Akt-mediated phosphorylation at Ser234. Beclin 1 pS234 Antibody is ideal for researchers interested in cancer research.
Gene Name: BECN1
-
基因ID
- 8678
-
NCBI登录号
- NP_001300927
-
UniProt
- Q14457
-
途径
- Autophagy
抗原
-
