BCL3 抗体 (C-Term)
Quick Overview for BCL3 抗体 (C-Term) (ABIN6655291)
抗原
See all BCL3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-gu4s
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Supplier
- Rockland
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原理
- BCL3 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human BCL3.
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纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-BCL3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human BCL3 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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anti-B-Cell CLL/lymphoma 3 (BCL3) (AA 300-400) antibody
BCL3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 3 (BCL3) (AA 362-454) antibodyBCL3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 3 (BCL3) (AA 248-277), (Middle Region) antibodyBCL3 适用: 人, 小鼠 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 3 (BCL3) (C-Term) antibodyBCL3 适用: 人 WB, ELISA, IHC, IP, IF, FACS, FM 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 3 (BCL3) (AA 82-341) antibodyBCL3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 3 (BCL3) (AA 1-446) antibodyBCL3 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 3 (BCL3) (AA 1-446) antibodyBCL3 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 3 (BCL3) antibodyBCL3 适用: 人 WB, ICC, DB 宿主: 小鼠 Monoclonal 2069E2a unconjugated
anti-B-Cell CLL/lymphoma 3 (BCL3) (AA 127-376) antibodyBCL3 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 3 (BCL3) (AA 249-277), (Center) antibodyBCL3 适用: 人, 小鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB23954 unconjugated
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应用备注
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Immunoprecipitation_Dilution: User Optimized
ELISA_Dilution: 5.0 μg/mL
Immunohistochemistry_Dilution: 1:200
Flow_Cytometry_Dilution: User Optimized
IF_Microscopy_Dilution: 10 μg/mL
Western_Blot_Dilution: 1.0 μg/mL
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说明
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Anti-BCL-3 Antibody has been tested in Western Blot, ELISA, and Immunofluorescence. Expect a band at 47.5 kDa in western blot using appropriate lysates. Positive control used recombinant BCL3 protein, HEK293T overexpressing BCL3 or U87 whole cell lysates at 1μg/mL in WB, and U87MG with PFA at 10μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- BCL3 (B-Cell CLL/lymphoma 3 (BCL3))
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别名
- BCL3
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背景
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Synonyms: rabbit anti-BCL3 antibody, rabbit anti-BCL-3 Antibody, BCL 3, B-cell lymphoma 3 protein, B-cell lymphoma 3 protein Antibody
Background: BCL3 belongs to the ANKRD family. The protein contains seven ankyrin repeats, which are closely related to those found in I kappa B proteins. BCL3 functions as a transcriptional co-activator, regulator of transcriptional activation, promotor of transcription, and inhibitor of nuclear translocation of NF-kappa B. It is a proto-oncogene identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. BCL3 contributes to the regulation of cell proliferation. Therefore, BCL3 may be associated with B-cell chronic lymphocytic leukemia, lymphoma, or other hematologic diseases. Anti-BCL3 Antibody is useful for researchers interested in Cancer Research, NF-Kappa B Pathway, and TNF Signaling Pathway.
Gene Name: BCL3
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基因ID
- 602
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NCBI登录号
- NP_005169
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UniProt
- P20749
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途径
- NF-kappaB Signaling, Maintenance of Protein Location, Protein targeting to Nucleus
抗原
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