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CD3 epsilon 抗体 (APC)

The APC-conjugated 小鼠 单克隆 anti-CD3 epsilon antibody (Clone HIT3a) (ABIN6655175) specifically detects CD3 epsilon in FACS, IHC 和 IP. The antibody is reactive with 人 samples.
Rockland
产品编号 ABIN6655175
Supplier Product No.: 200-326-n12
发货至: 中国
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Quick Overview for CD3 epsilon 抗体 (APC) (ABIN6655175)

抗原

See all CD3 epsilon (CD3E) 抗体
CD3 epsilon (CD3E)

适用

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小鼠

克隆类型

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单克隆

标记

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This CD3 epsilon antibody is conjugated to APC

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

克隆位点

HIT3a
  • Supplier Product No.

    200-326-n12

    Supplier

    Rockland

    原理

    CD3 Allophycocyanin Antibody

    交叉反应 (详细)

    Reactivity is observed against human CD3.

    纯化方法

    Allophycocyanin conjugated CD3 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD3.

    过滤

    Sterile filtered

    免疫原

    Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with CD3 antigen.

    亚型

    IgG2a kappa

    Labeling Ratio

    1-2
  • 应用备注

    Immunoprecipitation_Dilution: User Optimized

    Immunohistochemistry_Dilution: User Optimized

    Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

    说明

    Anti-CD3 is tested in Flow Cytometry and useful for Immunoprecipitation, Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.2 % BSA (w/v)

    Preservative: 0.09 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

    有效期

    6 months
  • 抗原

    CD3 epsilon (CD3E)

    别名

    CD3E

    背景

    Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e

    Background: CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

    Gene Name: CD3E

    基因ID

    916

    NCBI登录号

    NP_000724

    UniProt

    P07766

    途径

    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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